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高分辨率的集成微流体探针用于生物组织的质谱成像

Xiangtang Li¹, Hang Hu¹, Julia Laskin¹

¹Department of Chemistry, Purdue University, West Lafayette, IN, 47907, United States.

Analytica chimica acta

|October 12, 2023

概括

一个新的微流体探测器增强了纳米喷雾脱电喷雾电离 (纳米-DESI) 质谱图像 (MSI) 强大的,高分辨率的分子绘制生物组织. 这一进步使得样本中的生物分子能够更快,更详细地分析.

科学领域:

分析化学分析化学
质谱仪成像成像质谱仪成像
生物分子分析

背景情况:

纳米喷雾脱电喷雾电离 (nano-DESI) 是一种用于高分辨率分子成像的环境电离技术.
现有的纳米-DESI方法可以面临复杂的生物样本的稳定性和吞吐量方面的挑战.

研究的目的:

开发和验证一种新的集成微流体探头 (iMFP),用于增强的纳米-DESI质谱成像 (MSI).
为了实现更高的空间分辨率和提高生物组织分子成像的稳定性.

主要方法:

新型iMFP的设计,使用更小的通道和优化的采样端口配置.
利用iMFP进行小鼠子宫和脑组织部分的纳米-DESI MSI.
评估了新探测器的空间分辨率,灵敏度,耐用性和吞吐量.

主要成果:

新的iMFP实现了强大的组织成像,空间分辨率为8-10μm.
证明了高灵敏度,耐用性和吞吐量,使大组织部分的高分辨率成像成为可能.
成功完成了初始的高分辨率,高通量成像实验.

结论:

重新设计的iMFP显著提高了纳米-DESI MSI的稳定性和性能.
这一进步促进了生物样本中生物分子的高通量,细胞分辨率映射.
通过详细的分子成像,为了解生物系统开辟了新的途径.

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