快速电化学侧流装置用于检测 Δ9 - - 四大麻素
Wanwisa Deenin1, Nadine Wenninger2, Martin G Schmid2
1Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand; The Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok, 10330, Thailand.
Analytica chimica acta
|October 12, 2023
概括
一种新的电化学侧流测定 (LFA) 能够快速和灵敏地检测delta-9四大麻素 (THC). 这种便携式设备为大麻分析提供了准确的现场THC测定.
科学领域:
- 分析化学 分析化学
- 生物技术是生物技术.
- 法医科学 法医科学 法医科学
背景情况:
- 大麻含有400多种化合物,包括delta-9四大麻 (THC) 和大麻 (CBD).
- THC是主要的精神活性成分,需要严格监管医用大麻提取物.
- 快速准确的THC检测方法对于质量控制和监管合规至关重要.
研究的目的:
- 为快速检测THC开发一种敏感的电化学方法,与侧向流量试验 (LFA) 结合使用.
- 创建一个便携式和具有成本效益的设备,用于现场THC测定.
- 根据标准分析方法验证设备的性能.
主要方法:
- 设计了一种电化学LFA装置,将一个印电极集成到侧流带中.
- 单克隆THC抗体与铁碳酸合被用作电活性标签.
- 检测THC的依据是它与测试区中的大麻素2型 (CB2) 受体结合.
主要成果:
- 开发的设备实现了快速检测时间在6分钟内.
- 对THC的高度敏感的检测极限为1.30 ng/mL被证明.
- 在分析大麻提取物时,传感器显示了与高性能液态染色学 (HPLC) 相似的结果.
结论:
- 电化学LFA是一种灵敏,快速和便携的替代方案,用于现场THC测定.
- 这种方法提供了一个具有成本效益的解决方案,用于在各种样本中进行准确的THC分析.
- 该设备有助于有效监测医用大麻和大麻产品中的THC含量.
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