相关实验视频

Updated: Jul 13, 2025

A Nonsequencing Approach for the Rapid Detection of RNA Editing

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

确定正确的RNA编辑站点:历史,挑战和机会

Meng How Tan^1,2

¹School of Chemistry, Chemical Engineering and Biotechnology, Nanyang Technological University, Singapore 637459, Singapore.

Accounts of chemical research

|October 12, 2023

概括

腺至氨酸 (A-to-I) RNA编辑使转录组多样化并调节基因表达. 纳米孔测序提供了一种有前途的新方法,可以直接检测这些RNA编辑部位在原生RNA分子中.

科学领域:

分子生物学分子生物学
遗传学遗传学是一个
生物化学生物化学

背景情况:

由ADAR酶介导的腺酸到氨酸 (A-to-I) RNA编辑是改变RNA序列和多样化转录组的关键基因调节机制.
A-to-I编辑产生A-to-G核酸变化,影响蛋白质异型,RNA处理和先天免疫反应. 失调与包括癌症在内的人类疾病有关.
准确识别A-to-I编辑站点对于了解RNA生物学和疾病至关重要,但现有的测序技术仍然存在挑战.

研究的目的:

审查RNA测序技术的进步,以检测A-to-I编辑站点.
突出纳米孔测序对于直接检测RNA修饰的潜力.
为了强调在各种生物环境中识别含有氨酸的RNA的重要性.

主要方法:

对历史和当前的核酸测序技术 (Sanger,Illumina,Nanopore) 进行RNA编辑检测的审查.
讨论用于从测序数据中识别A-to-I编辑站点的计算方法和过器.
强调纳米孔测序对直接本源RNA分析的能力.

主要成果:

桑格测序提供了A-to-I编辑的早期,低吞吐量检测.
照明测序使高通量识别成为可能,但在短读数和绘图准确性方面面临挑战.
纳米孔测序显示了直接,原生RNA分析的潜力,克服了cDNA转换的局限性,并保留了基因修改.

结论:

技术进化显著改善了A-to-IRNA编辑站点的检测.
纳米孔测序代表了表体转录学研究的变革性技术,可以直接检测RNA修饰.
预计纳米孔技术的进一步开发将提高准确性,并扩大其在RNA编辑研究中的应用.

更多相关视频

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes

Published on: July 22, 2014

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

相关概念视频

RNA Editing

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...

Nonsense-mediated mRNA Decay

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...

Bacterial RNA Polymerase

Bacterial RNA Polymerase

Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...