在药用植物Phyllanthus emblica L. (Aonla) 中基于基因SSR的新型二次代谢物的新型转录组概况和开发
Bhuvnesh Kapoor1,2, Megha Sharma1, Rajnish Sharma3
1Department of Biotechnology, Dr YS Parmar University of Horticulture & Forestry, Nauni, Solan, HP, 173 230, India.
Scientific reports
|October 12, 2023
概括
这项研究开发了Phyllanthus emblica (印度) 的新遗传标记,以帮助改善作物. 这些EST-SSR与有价值的药物化合物的基因联系在一起,促进精英基因型选择.
科学领域:
- 基因组学就是基因组学.
- 植物科学 植物科学
- 生物技术是生物技术.
背景情况:
- 印度 (Phyllanthus emblica) 具有重要的药用特性,但其遗传结构仍未得到充分研究.
- 在P. emblica中,类和类等二次代谢物具有很大的药理学潜力.
- 了解这些化合物的遗传基础对于作物改进至关重要.
研究的目的:
- 为了组装P. emblica. 的转录组.
- 识别和开发与参与二次代谢物生物合成的基因相关的EST-SSR标记物.
- 为P. emblica繁殖和相关物种提供遗传资源.
主要方法:
- 使用Illumina RNASeq500平台进行转录组组.
- 通过BlastX.使用NCBI非冗余 (Nr) 数据库对 unigenes 的功能注释.
- 基于转录基因数据的EST-SSR标记物的设计和验证.
主要成果:
- 产生了超过3990万个高质量的读数,组装成126,606个转录和87,771个单基因.
- 鉴定了38692个爆炸性击中序列,与Hevea brasilensis (16%) 和Jatropha curcas (12%) 的最大相似性.
- 开发了30个EST-SSR原始序列,其中12个高度多态的标记物显示出显著的遗传多样性 (PIC=0.80).
结论:
- 新开发的EST-SSR与二次代谢物生物合成中的关键基因有关.
- 这些标记物代表了P. emblica作物改良的宝贵遗传资源.
- 促进精英基因型的选择在P.embblica和相关的Phyllanthaceae物种.
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