电子烟的哪些因素决定了消费者的口腔上皮细胞DNA损伤?
1Department of Oral and Maxillofacial Surgery, University Hospital Crosshouse, Kilmarnock Rd, Crosshouse, Kilmarnock, KA2 0BE, UK. siofra.murphy2@ggc.scot.nhs.uk.
Evidence-based dentistry
|October 12, 2023
概括
这项研究发现,电子烟和吸烟都会增加口腔细胞中的DNA损伤,较高的水平与使用量增加有关. 电子烟设备类型和风味也会影响从不吸烟者的DNA损伤水平.
科学领域:
- 口腔健康研究 口腔健康研究
- 毒理学 毒理学 毒理学
- 遗传学 是一个遗传学.
背景情况:
- 电子香烟 (vaping) 和传统香烟 (吸烟) 的使用在世界各地普遍存在.
- 了解这些习惯对细胞和分子的影响对于公共健康至关重要.
- 以前的研究表明,吸烟和电子烟都可能造成伤害,但需要直接比较DNA损伤.
研究的目的:
- 为了比较口腔上皮细胞中的DNA损伤水平,单独的电子烟者,单独的吸烟者和非吸烟者之间.
- 为了研究使用 (vaping或吸烟) 和DNA损伤之间的剂量依赖关系.
- 探索电子烟特征 (设备类型,口味) 对DNA损伤的影响.
主要方法:
- 招募了72名受试者,分为三个组:专属电子烟者,专属吸烟者和非吸烟者.
- 通过刷活检收集口腔上皮细胞样本.
- 使用LA-QPCR对POLB和HPRT基因的DNA损伤进行量化.
- 测量了血中丁胺,呼出的CO和%COHb,以评估暴露.
主要成果:
- 与非吸烟者相比,电子烟者的平均DNA损伤是吸烟者的2.6倍,吸烟者的2.2倍 (POLB基因).
- 随着电子烟液体体体积或吸烟包年数的增加,DNA损伤显示出剂量依赖的增加.
- 使用Pod设备的用户的DNA损伤增加了3.3倍;甜味的电子液体也与损伤增加相关.
结论:
- 证据支持吸烟者和吸烟者的口腔细胞中剂量依赖的DNA损伤形成.
- 电子烟设备类型和风味可能会显著影响观察到的DNA损伤水平.
- 研究结果强调了与vaping相关的潜在口腔健康风险,即使在从未吸烟的人中也是如此.
更多相关视频
09:30A Microcontroller Operated Device for the Generation of Liquid Extracts from Conventional Cigarette Smoke and Electronic Cigarette Aerosol
Published on: January 18, 2018
8.4K
12:15Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
Published on: May 29, 2019
8.8K
相关概念视频
Stimulants
224
Stimulants are substances that enhance neural activity and elevate dopamine levels in the brain, leading to their highly addictive nature. These drugs include cocaine, amphetamines, MDMA, caffeine, and nicotine, each with distinct mechanisms of action and varied health implications.
Cocaine can be administered via snorting, injection, or smoking. It primarily functions by blocking the reuptake of dopamine, resulting in a euphoric high characterized by an intense sensation of happiness and...
Cocaine can be administered via snorting, injection, or smoking. It primarily functions by blocking the reuptake of dopamine, resulting in a euphoric high characterized by an intense sensation of happiness and...
224
Cancer Prevention
6.2K
Several factors can increase the risk of cancer in an individual. About 50% of cancer cases can be prevented by adopting a healthy lifestyle, regular exercise, eating healthy, and following a modest cancer prevention diet. Epidemiological studies have consistently shown that populations with vegetable and fruit-rich diets have reduced the incidence of cancer. On the other hand, populations who have a diet rich in animal fat, red meat, junk food, or high calories are predisposed to cancer.
Some...
Some...
6.2K
Spontaneous and Induced Mutations
23
Spontaneous mutations arise infrequently during DNA replication due to errors in the process. A key factor behind these errors is tautomeric shifts in nitrogenous bases, where bases transition from keto to enol forms or amino to imino forms. This shift can alter base-pairing rules, leading to mutations. Additionally, reactive oxygen species (ROS) arising from aerobic metabolism can damage DNA, resulting in depurination (loss of a purine base) or depyrimidination (loss of a pyrimidine base).
23
Nucleotide Excision Repair
3.5K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
3.5K
Mutations
38.1K
Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
38.1K
DNA Damage can Stall the Cell Cycle
9.2K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.2K
