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对于花生 (Arachis Hypogaea L.) 叶的转录组和染色质可访问性的单核分辨率图谱

Hao Liu¹, Zenhua Guo², Sunil S Gangurde³

¹Guangdong Provincial Key Laboratory of Crop Genetic Improvement, South China Peanut Sub-Center of National Center of Oilseed Crops Improvement, Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong Province, 510640, China.

Advanced biology

|October 12, 2023

概括

这项研究创建了花生叶的单核地图,揭示了基因表达和染色体在发育过程中的可访问性动态. 主要发现包括乙烯-AP2模块.

科学领域:

植物生物学植物生物学
基因组学就是基因组学.
发展生物学发展生物学

背景情况:

花生是石油和蛋白质的全球重要作物.
了解花生叶发育中的基因表达和染色质可访问性至关重要,但仅限于单细胞分辨率.

研究的目的:

开发一个花生叶的单核地图.
在单细胞分辨率下同时描述转录组和染色质可访问性.
阐明控制花生叶子发育的动力机制.

主要方法:

单核RNA测序 (snRNA-seq) 和使用测序 (ATAC-seq) 测定转移酶可访问染色质的试验在单核中进行.
光激活分类用于单核隔离.
使用了生物信息分析,包括细胞聚类,发育轨迹分析和TF网络构建.

主要成果:

创建了一个包括5930个细胞和5315个可访问的染色体区域的花生叶图谱.
在叶子细胞分化中确定了乙烯-AP2模块.
昼夜节律,脂肪酸通路,细胞激素模块 (LHY/LOG) 和AT protein AhAHL11都与特定的发育过程有关.

结论:

同时对转录和染色质可访问性进行分析,为花生叶细胞发育提供了新的见解.
识别的监管模块和TF为改善花生叶的发展和作物产量提供了目标.

关键词:

染色质可访问性染色质可访问性地面核桃是如何使用的叶子的发育情况植物单细胞多组体.单个核的单个核.

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