新型条纹生效应器通过影响组织蛋白修饰来提高宿主易感因子的转录,从而促进小麦的感染
Wanlu Duan1, Zhenkai Hao1, Huihui Pang1
1College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, 712100, China.
The New phytologist
|October 13, 2023
概括
这项研究表明,真菌效应剂PstGTA1直接操纵小麦基因表达. PstGTA1针对宿主易感性基因促进体,增强病原体的毒性.
科学领域:
- 植物病理学 植物病理学
- 分子植物-微生物相互作用
- 菌类遗传学 菌类遗传学
背景情况:
- 植物病原体通常会改变宿主基因表达,从而导致疾病的发展.
- 转录活动的真菌效应体对宿主基因的直接操纵尚未得到充分理解.
研究的目的:
- 调查真菌病原体是否使用具有转录活性的分泌效应体来操纵宿主基因表达.
- 识别和描述一种来自Puccinia striiformis f. sp. 的新型真菌效应剂. 三 (Pst) 参与致病性.
主要方法:
- 确定了PstGTA1效应体,与酵母转录激活剂SNF2.2具有同质性.
- 在酵母中验证了PstGTA1转录活性.
- 使用转基因小麦评估PstGTA1在致病性中的作用.
- 通过转录基因分析选PstGTA1向基因.
- 确定了PstGTA1促进体结合部位和对基因素乙化的影响.
主要成果:
- PstGTA1具有转录激活活性.
- PstGTA1被输送到小麦核中,对Pst的毒性至关重要.
- PstGTA1的目标是TaSIG的促销者,这是一个小麦易感基因.
- PstGTA1可能通过改变基因组H3K4乙化来激活TaSIG转录.
结论:
- 这项研究提供了第一个证据,证明一种具有交换活化活动的真菌效应剂.
- PstGTA1通过促进体结合和基因素修饰直接调节宿主敏感性基因转录.
- 这种机制有助于小麦中Pst的毒性.
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