相关实验视频

Updated: Jul 13, 2025

Genotyping of Sea Anemone during Early Development

Genotyping of Sea Anemone during Early Development

Published on: May 13, 2019

微观的Nemertoderma westbladi的基因组草案揭示了Acoelomorpha基因组的演变

Samuel Abalde¹, Christian Tellgren-Roth², Julia Heintz²

¹Department of Zoology, Swedish Museum of Natural History, Stockholm, Sweden.

Frontiers in genetics

|October 13, 2023

概括

来自Xenacoelomorpha的第一个尼默特皮类基因组被测序,揭示了保存的基因组特征和缺少一个关键的分泌系统基因 (Osr). 这促进了对双边进化的理解,并突出了一个新的测序方法,用于挑战种类.

科学领域:

进化生物学进化生物学
基因组学就是基因组学.
海洋生物学海洋生物学

背景情况:

克塞纳科埃洛莫尔法是研究双边进化的关键海洋模型生物.
对于Xenacoelomorpha的基因组数据是有限的,特别是对于古老的Nemertodermatida类.
之前的研究缺乏Nemertodermatida的基因组资源,阻碍了进化见解.

研究的目的:

为了呈现第一个基因组序列为一个nemertodermatid物种.
分析Xenacoelomorpha和相关类的基因内容演变.
研究与分泌系统相关的基因的进化史.

主要方法:

从单个样本中提取DNA,然后进行PacBio HiFi测序.
基因组组装,消毒,注释和质量基准测试.
对各种动物类的基因含量进行比较基因组分析.

主要成果:

首个尼默特皮虫基因组 (Nemertoderma westbladi) 已经成功地被测序和注释.
在Acoelomorph的基因组中表现出保存的特征,许多基因在原生体/双生体中存在,而在Acoelomorpha中不存在.
在Xenacoelomorpha中,除了Osr之外,所有分泌系统基因都存在,与专门的分泌系统起源有关.

结论:

超低输入DNA协议和HiFi测序使单个动物的高质量基因组成为可能,即使对于具有挑战性的种类.
这项研究为比较基因组学和理解双边进化提供了有价值的基因组资源.
这些发现为Xenacoelomorpha的分泌系统的演变提供了新的见解.

关键词:

这就是HiFi,HiFi.美国的奥斯陆超低低的DNA输入这是Xenacoelomorpha.排泄系统的排泄系统基因内容基因内容

更多相关视频

Rapid Isolation of Wild Nematodes by Baermann Funnel

Rapid Isolation of Wild Nematodes by Baermann Funnel

Published on: January 31, 2022

Visualizing Bacteria in Nematodes using Fluorescent Microscopy

Visualizing Bacteria in Nematodes using Fluorescent Microscopy

Published on: October 19, 2012

相关概念视频

Diversity of Protists I

Diversity of Protists I

Excavata is a diverse group of protists that includes both chemoorganotrophic and phototrophic species, with some thriving in anaerobic environments. Among the key groups within Excavata are diplomonads and parabasalids, which are flagellated protists that lack mitochondria and chloroplasts. These microorganisms typically inhabit anoxic environments, such as the intestines of animals, where they exist either symbiotically or as parasites, relying on fermentation for energy production. Some...

Genome Size and the Evolution of New Genes

Genome Size and the Evolution of New Genes

While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.

Eukaryotic Evolution

Eukaryotic Evolution

The endosymbiont theory is the most widely accepted theory of eukaryotic evolution; however, its progression is still somewhat debated. According to the nucleus-first hypothesis, the ancestral prokaryote first evolved a membrane to enclose DNA and form the nucleus. Conversely, the mitochondria-first hypothesis suggests that the nucleus was formed after endosymbiosis of mitochondria.
Contrary to the endosymbiont theory, the eukaryote-first hypothesis proposes that the simpler prokaryotic and...

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...