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在人类心肌细胞的病理和生理性缩之间进行比较的全表观基因组DNA甲基化概况

Hangchuan Shi^1,2, Si Chen³, Fanju W Meng⁴

¹Department of Clinical and Translational Research, University of Rochester Medical Center, Rochester, NY, United States.

Frontiers in genetics

|October 13, 2023

概括

这项研究揭示了人类心脏细胞中不同的DNA甲基化模式,这些细胞对病理 (血管酶二) 和生理 (IGF-1) 刺激作出反应. 心脏缩中的这些表观遗传变化可以帮助区分疾病状态.

科学领域:

心血管生物学心血管生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子心脏病学分子心脏病学

背景情况:

心脏缩是由各种刺激引起的,但分子区别,特别是通过DNA甲基化进行表观遗传调节,仍然不清楚.
了解这些差异对于诊断和治疗心脏病至关重要.

研究的目的:

为了研究和比较全基因组的DNA甲基化模式在人类心肌细胞在病理 (血管新生素II) 和生理 (IGF-1) 缩条件下.
确定与不同形式的心脏缩相关的特定表观遗传标记和分子途径.

主要方法:

人类心肌细胞用血管新素II (病理) 和IGF-1 (生理) 进行治疗,以模拟心脏缩.
使用MethylationEPIC平台 (>850K位点) 进行了全基因组DNA甲基化分析.
进行了基因表达分析 (qRT-PCR) 和通路丰富分析.

主要成果:

在194个位点 (AngII) 和206个位点 (IGF-1) 观察到显著的差异性DNA甲基化.
相关的基因组揭示了PI3K-Akt通路对刺激和IGF-1的Hippo通路的丰富.
对于像CDK6和RPTOR这样的PI3K-Akt通路基因,观察到明显的甲基化模式,通过差异性基因表达得到证实.

结论:

这项研究提供了人类心肌细胞在明显的病理和生理过度缩下首次表观基因组范围内的DNA甲基化概况.
鉴定了DNA甲基化位置,基因和途径,提供了潜在的生物标志物,以区分病理和生理性心脏缩.

关键词:

在CDK6中使用CDK6.通过DNA甲基化.河马河马是什么意思海马河马是什么意思这就是PI3K-Akt.在RPTOR上,您可以使用RPTOR.心脏过度缩小的心脏过度缩小信号传输信号传输

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