SeqVerify:一种可访问的细胞系基因组完整性,污染和基因编辑结果分析工具
Merrick Pierson Smela1,2, Valerio Pepe1,2, George M Church1,3,4
1Wyss Institute at Harvard University, Cambridge, Massachusetts, United States of America.
bioRxiv : the preprint server for biology
|October 13, 2023
概括
SeqVerify是一个新的计算管道,它使用全基因组测序 (WGS) 来确认多能干细胞 (PSC) 中的基因组编辑. 它通过检测细胞系中的DNA异常和不需要的序列来确保实验的准确性.
科学领域:
- 基因组学就是基因组学.
- 干细胞生物学 干细胞生物学
- 生物信息学是一种生物信息学.
背景情况:
- 基因组编辑和多能干细胞 (PSC) 培养技术的进步使得编辑后的PSC细胞系可以用于生物研究.
- 细胞系异常,如形积分,意外的DNA编辑和污染,可能会损害实验有效性.
- 强有力的质量控制对于干细胞研究的可靠结果至关重要.
结论:
- SeqVerify提供了一个可访问的解决方案,用于编辑PSC的质量控制.
- 这种工具提高了使用工程细胞系的实验的可靠性.
- 它广泛适用于超越PSC的细胞线质量控制.
相关概念视频
02:54Evolutionary Relationships through Genome Comparisons
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
02:26Comparing Copy Number Variations and SNPs
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...