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标记器辅助早期代鉴定耐根结病的色番茄分离体,具有多个可取的等位基因

Awnish Kumar¹, Vijaya Lakshmi¹, Surabhi Sangam²

¹Department of Plant Breeding and Genetics, Bihar Agricultural College, Bihar Agricultural University, Sabour, Bhagalpur, Bihar 813210 India.

Physiology and molecular biology of plants : an international journal of functional plant biology

|October 13, 2023

概括

标记器辅助选择确定了优越的色果系列,具有增强的营养和抗病能力. 这种分子育种方法可以开发出具有理想特征的改良番茄基因型.

科学领域:

植物育种和遗传学
分子生物学分子生物学
园艺科学园艺科学

背景情况:

色果实番茄突变体积累了cis-lycopene异构体,增强了营养价值.
标记器辅助选择 (MAS) 便于对优质番茄基因型的可取代基因堆叠.

研究的目的:

用苗阶段MAS识别优越的,抗根结病的色果分离物.
为了验证疾病耐药性和评估水果质量特征在选定的隔离剂.

主要方法:

在146个F2植物上进行种植阶段标记器辅助选择 (MAS).
根结病耐药性的验证,使用实验室内根结的茎切片和人工注射.
果实特征的表征,包括重量,周骨厚度和可溶性固体总量.

主要成果:

确定了三种具有根结病耐药性的优异色果分离物.
选择的分离剂表现出水果重量 (39.254.6克),周骨厚度 (4.566.05毫米) 和可溶性固体 (3.654.87°Brix).
父母的多样性使得对抗其他疾病和理想的生长习惯的等位基因的鉴定成为可能.

结论:

选择的分离剂为开发多种抗病,营养丰富的果番茄系列提供了基础.
早期生成的MAS是有效的准确准和固定多个可取的等位基因在番茄育种.
这项研究表明,MAS对于开发优异的番茄基因型具有更好的水果质量和抗病能力的实用性.

关键词:

胡卜素的生物利用性胡卜素的生物利用性水果质量水果的质量.标记器辅助的选择梅洛伊多基尼菌种 (Meloidogyne spp.) 是一种植物.对多种疾病的耐药性.菜突变的突变种子

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