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通过CLA-ICP-MS和单细胞纳米喷雾HRMS在西兰花细胞中识别和定位不稳定分子

Changzhi Shi^1,2, Fan Li¹, Hetian Jia¹

¹State Key Laboratory of Biogeology and Environmental Geology, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan 430074, P. R. China.

Journal of the American Society for Mass Spectrometry

|October 13, 2023

概括

研究人员开发了一种新方法,在单细胞中分析脆弱的植物化合物,如葡萄糖酸盐 (GLs). 这项技术精确地定位和识别了植物组织中的这些防御分子,克服了以前在质谱分析中的局限性.

科学领域:

植物生物化学植物生物化学
分析化学是一种分析化学.
分子成像学分子成像学

背景情况:

像葡萄糖酸盐 (GLs) 这样的不稳定分子是关键的植物防御化合物,但很难使用传统质谱在单细胞水平上进行分析.
在组织损伤时GLs的快速降解使精确的定位和识别变得复杂,特别是在需要大量样本准备的表面成像技术中.

研究的目的:

开发一种新的策略,用于单个植物细胞中可变葡萄糖酸盐 (GLs) 的直接分析和成像.
克服传统质谱学的局限性,以单细胞分辨率分析快速降解的分子.

主要方法:

结合的冷却激光除诱导合质谱 (CLA-ICP-MS) 用于初始组织微区定位,用毛细微样本纳米喷射高分辨率质谱 (HRMS) 进行详细分析.
利用/交换反应与HRMS相结合,用于单细胞内精确识别三个特定的GL.
采用细胞对细胞纳米喷雾HRMS成像来绘制GL丰富细胞的分布.

主要成果:

CLA-ICP-MS成功地发现了富含硫的微区,这表明了富含GL的细胞群.
纳米喷雾HRMS在单细胞中准确识别了三种葡萄糖酸盐 (GL).
细胞对细胞成像揭示了GL丰富的细胞,位于茎表面以下约30微米.

结论:

开发的策略能够准确地识别和定位植物组织中单细胞中的像GLs这样的不稳定化合物.
这种方法克服了与样本降解相关的挑战,并为分析富含分子的细胞提供了强大的工具.
该方法有可能在识别和定位各种生物组织中其他性分子方面得到更广泛的应用.

关键词:

葡萄糖酸盐是什么葡萄糖酸盐影像成像技术影像成像技术在现场 (in situ) 进行.质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量一个单细胞的单细胞.

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