在乳腺癌细胞中灭介导的巨 (Agaricomycetes) 提取物的抗癌活性
Riji E1, Prashantha Naik1, Katheeja Muhseena N2
1Department of Biosciences, Mangalore University, Mangalagangothri - 574 199, Karnataka, India.
International journal of medicinal mushrooms
|October 13, 2023
概括
甘巨型提取物显示出对乳腺癌细胞的抗癌潜力. 乙酸乙烯提取物诱导了细胞亡和细胞循环停止,这表明它可能是新的癌症药物的来源.
科学领域:
- 菌类学 菌类学是指菌类学.
- 生物化学 生物化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 癌症是全球主要的健康问题,现有的治疗方法会导致副作用和复发.
- ,像Ganoderma colosus一样,正在探索新的治疗化合物.
- 需要新的抗癌药物,其疗效提高,毒性降低.
研究的目的:
- 为了评估Ganodermacolossus提取物对MCF-7乳腺癌细胞的抗癌活性.
- 为了阐明活性G.colossus提取物的作用机制.
- 为了识别G.colossus的潜在治疗分子用于乳腺癌治疗.
主要方法:
- 用MTT测定来评估细胞毒性.
- 分析了基因表达 (TP53,Bcl-2) 和DNA损伤 (H2AX焦点).
- 进行了细胞循环停止,细胞亡 (AO/EB染色,Caspase 3激活) 和化合物识别 (GC-MS).
主要成果:
- G. colossus 的乙烯酸提取物对 MCF-7 细胞表现出显著的细胞毒性活性 (IC50 = 17.2 ± 2.7).
- 提取物处理诱导了DNA损伤,G1/G0细胞循环停止和亡.
- GC-MS分析表明,提取物中存在诱导亡的化合物.
结论:
- 甘多德玛巨人乙烯酸提取物具有针对乳腺癌细胞的显著抗癌特性.
- 提取物诱导细胞亡和细胞循环停止,突出其治疗潜力.
- 需要进一步的研究来分离和描述造成这些影响的特定化合物.
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