脏特异性膜结合的血清蛋白酶CAP1/Prss8和CAP3/St14影响ENaC子单元的丰度,但不影响其活动
Elodie Ehret1,2, Sévan Stroh1, Muriel Auberson1
1Department of Biomedical Sciences, Faculty of Biology and Medicine, University of Lausanne, 1011 Lausanne, Switzerland.
Cells
|October 13, 2023
概括
血清蛋白酶CAP3 / St14调节表皮质通道 (ENaC) 蛋白水平,而不是其激活. 这一发现揭示了蛋白酶在ENaC表达和平衡中的复杂作用.
科学领域:
- 生理学 生理学 生理学
- 分子生物学分子生物学
- 脏生理学 脏生理学
背景情况:
- 已知血清蛋白酶CAP1/Prss8和CAP3/St14可以在体外激活ENaC.
- 它们在脏ENaC激活和吸收中的in vivo作用,特别是在剥夺期间,仍然不清楚.
研究的目的:
- 调查CAP3/St14在脏ENaC激活和缺乏下吸收中的作用.
- 为了确定CAP3/St14是否在体内对ENaC的蛋白质分解激活或蛋白质丰度至关重要.
主要方法:
- 使用了CAP3/St14淘汰赛小鼠和双CAP1/Prss8;CAP3/St14缺乏的小鼠.
- 评估了和平衡,ENaC子单元和pNCC蛋白质丰度以及NCC活性.
- 在管中使用RNAscope进行蛋白酶和ENaC子单元的共同表达分析.
主要成果:
- CAP3/St14缺乏导致CAP1/Prss8减少,改变了ENaC子单位,并减少了PNCC,但保持了平衡.
- 双缺陷恢复了ENaC亚单元水平,但在缺期间降低了NCC活性.
- 在远端管道中观察到CAP3/St14和CAP1/Prss8与alpha ENaC的同时表达.
结论:
- CAP3/St14对于ENaC蛋白的丰富性至关重要,而不是其直接的蛋白质分解活性.
- 这些血清蛋白酶表现出ENaC的复杂调节,主要是在表达水平.
- 这项研究阐明了CAP3/St14在通过ENaC调节维持平稳中的不同作用.
更多相关视频
10:08Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting
Published on: December 9, 2022
2.2K
08:46Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium
Published on: September 1, 2015
9.8K
相关概念视频
01:30Antihypertensive Drugs: Angiotensin-Converting Enzyme Inhibitors
677
Angiotensin-converting enzyme (ACE), a vital component of the renin-angiotensin-aldosterone system, is abundant in lung endothelial cells. ACE converts the inactive decapeptide, angiotensin I, into the active octapeptide, angiotensin II. This potent vasoconstrictor narrows blood vessels, increasing resistance to blood flow and elevating blood pressure. Angiotensin II also stimulates aldosterone production, encouraging kidney cells to reabsorb more sodium and water from urine, thereby increasing...
677
01:19Allosteric Proteins-ATCase
5.8K
Binding sites linkages can regulate a protein's function. For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
5.8K
01:27Transducer Mechanism: Enzyme-Linked Receptors
2.5K
Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
Major types that are helpful drug targets include:
2.5K