一种高效和具有成本效益的方法来产生功能的人类可诱导的多能干细胞衍生天体细胞
Hemil Gonzalez1,2, Srinivas D Narasipura2, Tanner Shull2,3
1Department of Internal Medicine, Division of Infectious Diseases, Rush University Medical Center, Chicago, IL 60612, USA.
Cells
|October 13, 2023
概括
对于培养人类诱导的多能干细胞衍生天体细胞 (iAs),Matrigel不需要. 这项研究表明,IA在没有Matrigel的情况下是可行的和功能性的,简化了它们用于研究的生成.
科学领域:
- 神经科学是一个神经科学.
- 干细胞生物学 干细胞生物学
- 细胞生物学 细胞生物学
背景情况:
- 人类诱导的多能干细胞衍生天体细胞 (IA) 是研究神经疾病的宝贵模型.
- 目前用于iAs生成的协议需要Matrigel涂层,限制可访问性和增加成本.
研究的目的:
- 开发和验证一种用于培养人类IA的无Matrigel协议.
- 证明没有Matrigel培养的IA保持了它们的特征性质.
主要方法:
- 人类IA被培养成Matrigel和没有Matrigel.
- 综合性表征包括形态学,细胞特异性标记,基因表达,代谢活性和功能性检测 (谷氨酸摄取,细胞因子反应).
主要成果:
- 没有Matrigel培养的人类IA表现出生存能力和繁殖.
- 与Matrigel培养的IA相比,在形态学,表型,基因型,代谢功能或功能反应方面没有发现显著差异.
- 与其他中枢神经系统细胞类型不同,iAs对Matrigel缺失表现出弹性.
结论:
- 对于人类iPSC衍生天体细胞的成功培养,Matrigel是不可或缺的.
- 这一发现为研究应用生成IA提供了一种简化,成本效益和精简的方法.
相关概念视频
00:58Embryonic Stem Cells
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
01:27Source And Potency Of Stem Cells
Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...
00:57Embryonic Stem Cells
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
01:22iPS Cell Differentiation
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
01:28Forced Transdifferentiation
Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial transdifferentiation occurs...
Artificial transdifferentiation occurs...
01:24Methods of Nuclear Reprogramming
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.