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Combinatorial Gene Control02:33

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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
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Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
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一个用于CPEB介导的c-myc压制的组合代码

Koichi Ogami1,2, Keima Ogawa1, Shoko Sanpei1

  • 1Department of Biological Chemistry, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan.

Cells
|October 13, 2023
PubMed
概括
此摘要是机器生成的。

细胞质多基化元素结合蛋白 (CPEB) 抑制体细胞中c-myc mRNA的翻译. 共识 (cCPE) 和非共识 (ncCPE) 元素的特定组合决定了CPEB结合和c-myc mRNA衰变.

关键词:
在CPEB中,CPEB是CPEB.这是一种RNA结合蛋白.在mRNA死亡化过程中.

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科学领域:

  • 分子生物学分子生物学
  • 关于RNA的规则 关于RNA的规则
  • 基因表达 基因表达

背景情况:

  • 在胚胎发育过程中,细胞质多基化元素结合蛋白 (CPEB) 调节mRNA翻译.
  • 在非神经元体细胞中,CPEB的作用通过不清楚的机制转移到抑制翻译,包括c-myc mRNA.
  • 对于目标mRNA调节,CPEB使用了细胞质多化元素 (CPE) 的组合代码.

研究的目的:

  • 阐明体细胞中CPEB介导的c-myc mRNA衰变的cis调节机制.
  • 定义控制CPEB结合和随后的mRNA降解的CPE组合代码.

主要方法:

  • 用RNA突变发生法来识别关键的CPE序列.
  • 使用电泳运动转移试验 (EMSA) 来量化CPEB结合亲缘关系.
  • 进行了mRNA降解试验,以评估CPE组合的功能影响.

主要成果:

  • 协同共识 (cCPE) 和非共识 (ncCPE) 元素的组合形成了CPEB介导的c-myc mRNA衰变的特定代码.
  • CPEB表现出对cCPE的高亲和度结合 (~250nM) 和对ncCPE的低亲和度结合 (>900nM).
  • 与cCPE的结合增强了随后的CPEB与ncCPE的结合,ncCPE对于降解诱导至关重要,并通过cCPE的存在进一步促进.

结论:

  • 提出了一个模型,其中高亲和度的CPEB与cCPE的结合促进了对ncCPE的二次结合.
  • 这种合作结合事件导致死乙酶招募,加速死乙和抑制c-myc mRNA翻译.
  • 对于调节mRNA衰变中CPEB的功能来说,CPE的组合代码至关重要.