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在口腔状细胞癌中绘制细胞内的细胞结构

Leonardo de Oliveira Siquara da Rocha^1,2, Bruno Solano de Freitas Souza^1,3, Ricardo Della Coletta^4,5

¹Gonçalo Moniz Institute, Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador 40296-710, BA, Brazil.

|October 13, 2023

概括

用3D生物打印模型研究了口腔状细胞癌 (OSCC) 球体中的细胞内细胞结构. 这些结构主要存在于与癌症相关的混合纤维细胞球体中,可能会影响瘤的攻击性.

科学领域:

在瘤学瘤学.
细胞生物学细胞生物学
生物技术是生物技术.

背景情况:

细胞内细胞 (CIC) 结构与侵袭性口腔状细胞癌 (OSCC) 和患者的不良结果有关.
了解OSCC中CIC形成的分子机制需要先进的体外模型.

研究的目的:

使用3D球形模型研究口腔状细胞癌 (OSCC) 中的细胞内细胞 (CIC) 结构.
分析瘤微环境中的CIC结构的形成,形态和位置.

主要方法:

口腔状细胞癌 (OSCC) 和癌症相关纤维细胞 (CAF) 细胞系使用生物打印系统培养成球形.
用显微镜和图像分析软件,随着时间的推移,分析了球状体形态,包括缺氧和增殖区.
在不同的球状区域内评估了细胞内细胞结构的发生,复杂性和形态.

主要成果:

在6小时内形成了模仿瘤微环境 (低毒核心,增殖外围) 的形成良好的球状体.
在同型和异型球形体中观察到细胞内细胞 (CIC) 结构.
主要在混合OSCC/CAF球体的繁殖区发现了CIC结构,并指出了"复杂食人主义"的情况.

结论:

3D球形模型有效地复制了OSCC瘤微环境的关键特征,包括CIC形成.
该模型为进一步研究OSCC中CIC结构的形态学,发展和预后意义提供了一个有价值的平台.

关键词:

与癌症相关的纤维细胞.细胞食人主义细胞食人细胞内的细胞结构.口腔状细胞癌的癌症.瘤球体是瘤球体.

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