在雨水系统中检测和减轻非法连接的低技术和高技术方法之间的比较研究
Remy Schilperoort1, Johan Post2, Martijn Klootwijk3
1Partners4UrbanWater, Nijmegen, The Netherlands
概括
检测非法废水连接需要先进的方法. 虽然一些标记物,如大肠杆菌在修复后没有足够的特异性,但人类废水DNA标记物 (HF183) 显著减少,证实了成功的缓解.
科学领域:
- 环境科学 环境科学
- 水质管理水质管理
- 微生物学 微生物学
背景情况:
- 非法将废水连接到雨水系统导致未经处理的排放,污染水生环境.
- 独立的下水道系统容易受到这些错误连接的影响,因此需要有效的检测方法.
研究的目的:
- 同时评估多种低科技和高科技方法来检测非法废水连接.
- 通过使用各种生物和基于DNA的标记来评估缓解努力的有效性.
主要方法:
- 网状屏幕的应用,电导性,温度,大肠杆菌 (大肠杆菌) 和扩展光谱β-乳糖酶产生大肠杆菌 (ESBL-EC) 的分析.
- 定量聚合酶连锁反应 (qPCR) 对于人类 (HF183),狗和鸟类便的指标.
- 分布式温度传感 (DTS) 用于水文评估.
主要成果:
- 所有经过测试的方法都成功地发现了重要的非法联系.
- 低技术方法的检测效率受到液压条件和污水量的影响.
- 缓解后,大肠杆菌和ESBL-EC水平没有显著变化,但人类特异性DNA标记物 (HF183) 显著下降.
结论:
- 人类特异性DNA标记物 (HF183) 比一般大肠杆菌或ESBL-EC更有效地评估非法连接缓解成功.
- 方法的组合提供了全面的检测,但标记物的特异性对于评估修复效率至关重要.
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