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相关概念视频

RNA Editing02:23

RNA Editing

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
9.4K
Riboswitches01:56

Riboswitches

8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
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Protein Modifications in the RER01:26

Protein Modifications in the RER

5.2K
Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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相关实验视频

Updated: Jul 13, 2025

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants
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A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

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PRMD:植物RNA修饰的综合数据库.

Xiaoqiang Lang1,2, Chunyan Yu3, Mengyuan Shen4

  • 1State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.

Nucleic acids research
|October 13, 2023
PubMed
概括

研究人员开发了植物RNA修饰数据库 (PRMD),以集中植物RNA修饰数据,包括N6-甲基氨酸 (m6A). 本资源有助于理解RNA在植物生理学和病理学中的作用.

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2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
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mRNA Interactome Capture from Plant Protoplasts
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相关实验视频

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2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
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科学领域:

  • 植物分子生物学 植物分子生物学
  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 对于植物生理和病理过程来说,RNA的修饰,特别是N6-甲基氨酸 (m6A),至关重要.
  • 这些修改影响RNA结构,影响分子相互作用和蛋白质结合.
  • 越来越多的研究兴趣需要为植物RNA修饰数据提供综合资源.

研究的目的:

  • 开发一个全面和用户友好的植物RNA修改数据库.
  • 为研究人员提供综合数据和可视化工具.
  • 为了促进对20种植物的RNA修饰的研究.

主要方法:

  • 开发植物RNA修饰数据库 (PRMD).
  • 包括20种植物物种的数据.
  • 功能分析工具 (RMlevelDiff,RMplantVar,RNAmodNet,Blast) 和数据可视化工具 (mRNAbrowse,RNAlollipop,JBrowse,IGV) 的整合.

主要成果:

  • PRMD提供了一个直观的界面来访问和显示植物RNA修饰信息.
  • 该数据库包括20种不同的植物物种的数据.
  • 在PRMD中集成了多种分析和可视化工具.

结论:

  • PRMD为植物RNA修饰研究提供了一个有价值的,免费可访问的资源.
  • 这个数据库将加速植物RNA修饰的开发和理解.
  • PRMD支持植物RNA生物学中的功能分析和数据探索.