mHapBrowser:用于可视化和分析DNA甲基化单元型的全面数据库.
Yuyang Hong1, Leiqin Liu1, Yan Feng1
1Key Laboratory of RNA Science and Engineering, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai 200031, China.
Nucleic acids research
|October 13, 2023
概括
mHapBrowser可视化了DNA甲基化哈普洛类型 (mHaps),提供了超出平均甲基化的新见解. 这个数据库有助于理解基因表达和疾病分类,使用阶段甲基化模式.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因组学就是基因组学.
- 生物信息学是一种生物信息学.
背景情况:
- 基因甲基化是基因表达的关键表观遗传调节剂.
- 双硫酸盐测序提供甲基化的单基解.
- 目前的数据库缺乏对阶段甲基化模式 (mHaps) 的分析.
研究的目的:
- 介绍mHapBrowser,这是一个可视化和分析DNA甲基化单体类型 (mHaps) 的数据库.
- 提供工具来比较不同样本的mHap模式.
- 促进对mHap数据的下游分析.
主要方法:
- 系统地处理来自人类,小鼠和老鼠组织的公开二硫酸盐测序数据.
- 为6366个样本生成mHap格式的文件.
- 与WashU Epigenome浏览器集成用于可视化和在线服务器进行比较.
主要成果:
- mHapBrowser 可视化八个mHap指标并进行样本之间的比较.
- 证明了mHap模式与基因表达之间的关联.
- 展示了mHap模式在肺癌亚型差异表达中的作用,并从无细胞DNA中改进了瘤分类.
结论:
- mHapBrowser为探索DNA甲基化单体类型提供了一个宝贵的资源.
- 阶段性甲基化模式提供了超出平均甲基化水平的洞察力.
- mHap分析在基因调节研究和疾病诊断中具有潜在的应用.
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