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Nitric Oxide Signaling Pathway01:28

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Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure...
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氧化诱导细菌的形态变化

Huan K Nguyen1, Samantha L Picciotti1, Magdalena M Duke1

  • 1Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States.

ACS infectious diseases
|October 13, 2023
PubMed
概括
此摘要是机器生成的。

氧化 (NO) 捐赠者损害了阴性细菌的细胞壁,而缓释捐赠者会造成更大的伤害. 需要带正电荷的树枝状体才能穿透格拉姆阳性细菌的细胞壁.

关键词:
逃离病原体的可能释放NO的树脂分子抗微生物耐药性 抗微生物耐药性形态变化 变化 形态变化氧化氧化的使用方法带正电荷的四分位氨四.

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科学领域:

  • 微生物学 微生物学
  • 生物化学 生物化学
  • 材料科学 材料科学 材料科学

背景情况:

  • 抗菌素耐药性是一个全球性的健康危机,需要新的治疗策略.
  • 氧化 (NO) 在病原体反应中发挥作用,并具有潜在的抗菌应用.
  • 外源的NO捐赠者为开发新的抗感染治疗提供了一个有希望的途径.

研究的目的:

  • 为了研究由各种外源性捐赠者输送的氧化 (NO) 对细菌细胞包裹的影响.
  • 评估NO供体架构如何影响格兰阴性和格兰阳性病原体中的细菌壁退化.
  • 评估不同NO输送系统对抗抗生素耐药菌株的疗效.

主要方法:

  • 使用基于显微镜和光的技术,在NO处理后检查细菌细胞包裹.
  • 传输电子显微镜 (TEM) 可视化了细菌细胞壁和膜的结构变化.
  • 脱极化试验测量了细胞壁透率作为对NO捐赠者的反应.

主要成果:

  • 没有捐赠者导致显著的膜损伤和细胞壁破坏在格拉姆阴性细菌 (Klebsiella pneumoniae,Pseudomonas aeruginosa).
  • 从小分子中缓慢释放的NO (t1/2 = 120分钟) 与快速释放 (t1/2 = 24分钟) 相比,导致格拉姆阴性细菌受到更大的损害.
  • 无治疗没有显著改变格拉姆阳性细菌 (黄金葡萄球菌,菌菌) 形态,但QA修饰的树状体穿透了它们的细胞壁.

结论:

  • 无氧化物供体的结构显著影响它们对细菌细胞包裹的有效性.
  • 缓慢释放的NO捐赠者在降解格拉姆阴性细菌细胞壁方面更有效.
  • 带正电荷的NO释放型树枝状体具有独特的潜入格兰正细菌的糖层的能力,为治疗这些感染提供了潜在的策略.