在原型设备中,电膜提取和液相微提取的提取性能
Maria Schüller1, Frederik André Hansen1, Stig Pedersen-Bjergaard2
1Department of Pharmacy, University of Oslo, P.O. Box 1068 Blindern, 0316 Oslo, Norway.
Journal of chromatography. A
|October 13, 2023
概括
液相微提取 (LPME) 和电膜提取 (EME) 进行了比较,用于从血中提取基本物质. EME 显示出更快的动力学,特别是对于具有中度脂友性的物质 (log P 2.0-4.0).
科学领域:
- 分析化学 分析化学
- 分离科学 分离科学
- 生物分析是一种生物分析.
背景情况:
- 液相微提取 (LPME) 和电膜提取 (EME) 是生物分析中重要的样本准备技术.
- 在商业瓶式设备中对LPME和EME进行标准化比较对于方法选择至关重要.
- 了解这些技术的性能特征对于优化从等离子体等复杂矩阵中提取分析物至关重要.
研究的目的:
- 为了比较评估基于小瓶的液相微提取 (LPME) 和电膜提取 (EME) 的性能,用于从血中提取基本分析物.
- 评估瓶子大小和脂性 (log P) 对LPME和EME的提取效率和动力学的影响.
- 调查EME中载体介导提取的潜力,以分析具有较低的脂友性.
主要方法:
- 使用具有导电瓶的商业电膜提取装置对LPME和EME进行比较分析.
- 从pH调整的血样本中提取90种基本物质,通过有机液体膜将其提取到酸性受体溶液中.
- 对两种技术的提取回收,动力学,线性,精度,准确性和矩阵效应的评估,包括对小 (200μL) 和大 (600μL) 瓶子格式的比较.
主要成果:
- 无论是LPME还是EME都有效提取了具有2.0
- 与LPME相比,电膜提取表现出明显更快的提取动力学.
- 较小的瓶体量 (200μL) 显著改善了LPME和EME的提取动力学.
- 载体介导的EME增强了对log P < 2的分析物的提取,尽管注意到了系统的不稳定性.
结论:
- 基于瓶子的LPME在生物分析应用中表现出有前途的性能,这项研究标志着其首次在商业瓶子设备中使用.
- 在某些应用中,EME提供更快的提取动力学,使其成为潜在的更高效的技术.
- 在LPME和EME之间选择方法时,应考虑分析物的脂友性,所需的提取时间和矩阵特征.
- 瓶子尺寸的优化对于提高LPME和EME的动力性能至关重要.
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