多镇压复合体2在成年嗅觉神经发生过程中调节基底细胞命运
Tiffany Ko1, Rhea Choi2, Khalil Issa2
1Department of Neurobiology, Duke University School of Medicine, Durham, NC 27710, USA.
Stem cell reports
|October 13, 2023
概括
多镇压复合体2 (PRC2) 通过控制干细胞谱系决策来调节成年嗅觉神经发生. 失去PRC2会破坏神经元的产生,并改变嗅觉表皮中的细胞命运.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 遗传学 是一个遗传学.
背景情况:
- 哺乳动物嗅觉上皮质中的成年神经发生对于维持嗅觉功能至关重要.
- 嗅觉基底干细胞在受伤后再生神经元,但血统控制机制尚不清楚.
- 多镇压复合体 (PRCs) 是参与发育的表观遗传调节者.
研究的目的:
- 研究Polycomb抑制复合体2 (PRC2) 在成人嗅觉神经发生中的作用.
- 了解PRC2如何影响受伤后嗅觉基础干细胞的血统决定.
- 为了确定PRC2-调节的基因和参与嗅觉神经元再生的途径.
主要方法:
- 使用了嗅觉上皮的小鼠病变模型.
- 使用有条件的PRC2功能丧失策略.
- 分析了基因组修饰,基因表达和细胞命运标记.
主要成果:
- PRC2蛋白在嗅觉基底细胞和发育中的神经元中表达.
- 有条件的PRC2损失影响了损伤诱导的神经发生.
- PRC2 缺乏导致基底细胞中转录因子的改变和错误表达.
- 在PRC2-突变细胞中抑制Sox9与增加的曼腺生产相关,揭示了细胞命运调节中的作用.
结论:
- 在成年嗅觉神经中,PRC2在促进感觉神经元分化方面发挥着至关重要的作用.
- 依赖PRC2的表观遗传机制调节了嗅觉表皮中的神经元和腺体之间的血统选择.
- 这些发现阐明了PRC2在成年干细胞命运决定中的未知的功能.
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