在配方的有机溶剂中菌体的稳定性
Yue Cao1, Dipesh Khanal1, Jinhee Kim2
1Advanced Drug Delivery Group, Sydney Pharmacy School, Faculty of Medicine and Health, The University of Sydney, Sydney, New South Wales, NSW 2006, Australia.
International journal of pharmaceutics
|October 13, 2023
概括
在有机溶剂中的菌体 (菌体) 稳定性对于开发针对多药耐药性感染的替代疗法至关重要. 这项研究发现,菌体在酒精中比DMSO或THF更稳定,而肌病毒比Podovirus更强壮.
科学领域:
- 生物技术和制药科学 生物技术和制药科学
- 微生物学和病毒学
- 药物输送和配方 药物输送和配方
背景情况:
- 菌体 (菌体) 是治疗多药耐药性感染的有希望的替代品.
- 菌体配方的开发通常涉及有机溶剂,但它们对菌体稳定性的影响尚不清楚.
- 目前的菌体配方策略中的实证实践需要对溶剂效应的科学理解.
研究的目的:
- 研究常见有机溶剂对Pseudomonas aeruginosa特定菌体稳定性的影响.
- 评估溶剂度和类型对菌体活力和形态学的影响.
- 为优化菌体配方策略提供数据驱动的见解.
主要方法:
- 在乙醇,异醇,THF和DMSO中测试了肌肉病毒 (PEV1,PEV20) 和 podovirus (PEV31) 的稳定性,其稳定性为25%,50%和75% (v/v).
- 使用生物测试 (标位损失) 评估菌体活力.
- 使用传输电子显微镜 (TEM) 和近场光学显微镜 (SNOM) 分析了菌体形态和蛋白质变化.
主要成果:
- 菌体在25%的溶剂度下保持活力,标位变化微不足道.
- 在50%度下,肌性病毒的稳定性高于podovirus,PEV31的标题损失显著.
- 较高度 (75%) 和DMSO和THF等溶剂导致形态损伤增加和生命力丧失;酒精的危害较小.
结论:
- 在有机溶剂中的菌体稳定性是菌体和溶剂依赖的;肌病毒通常比podoviruses更稳定.
- 与DMSO和THF相比,酒精显示出更好的菌体兼容性.
- 低至中度度 (25-50%v/v) 的特定有机溶剂可以在菌体配方中使用,而不会造成显著的标位损失.
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