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ROR2的同质化足以激活神经元中的Wnt/信号通路

Raul Riquelme¹, Laura Li², Abigail Gambrill¹

¹Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, Washington, USA.

The Journal of biological chemistry

|October 13, 2023

概括

之前认为是伪激酶的Wnt受体ROR2在神经元中积极发出信号. 它的同质化调节NMDA受体的贩运和神经元功能,独立于Frizzled受体.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
细胞信号传递细胞信号传递

背景情况:

对于成熟的中枢神经系统 (CNS) 功能来说,Wnt信号传递至关重要.
它调节NMDA类型的谷氨酸受体贩运和神经元特性.
ROR2是一种Wnt受体,与神经元Wnt5a/Ca2+信号通路有关.

研究的目的:

研究神经元中Wnt受体ROR2的信号机制.
为了确定ROR2是否可以独立于Frizzled受体发出信号.
阐明ROR2在调节突触功能中的作用.

主要方法:

在神经元中,ROR2的化学诱导同质化.
对蛋白质激酶C (PKC) 和c-Jun N-终端激酶 (JNK) 激活的测试.
测量细胞内 (Ca2+) 水平.
对NMDA受体贩运到突触的分析.
在ROR2 ATP结合部位的局部导向突变发生.

主要成果:

通过ROR2的同质化激活PKC和JNK激酶.
同体化增加了体和树突的Ca2+水平.
ROR2激活增强了NMDAR向突触的贩运.
同位体化诱导了ROR2氨酸酸化,这取决于它的ATP结合部位.
ROR2信号传递独立于Frizzled受体发生.

结论:

ROR2具有活性激酶域,可以独立于Frizzled受体发出信号.
同位体化足以激活ROR2下游信号通路.
ROR2调节NMDAR的贩运,这对成熟的中枢神经系统中的突触功能至关重要.
了解ROR2信号是理解成熟大脑功能的关键.

关键词:

这种NMDA受体是NMDA受体.在ROR受体中,ROR受体具有在Wnt路径上.没有信号发送.细胞信号传递是细胞的信号传递.葡萄糖酸盐受体的受体是什么神经生物学神经生物学神经生物学神经生理学神经生理学神经科学神经科学受体氨酸激酶的受体.

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