在严重的COVID-19中增加了血液免疫调节细胞,具有对I型干扰素的自身抗体
Fatemeh Saheb Sharif-Askari1,2, Narjes Saheb Sharif-Askari1,3, Shirin Hafezi1
1Research Institute for Medical and Health Science, University of Sharjah, Sharjah, UAE.
调节性T (Treg) 和B (Breg) 细胞在严重的COVID-19中发生变化,特别是与I型IFN的自身抗体发生变化. 较高的Treg/Breg水平与减少炎症和改善某些治疗结果相关.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 细胞生物学 细胞生物学
背景情况:
- 严重的COVID-19的特征是由于免疫功能障碍导致的不受控制的炎症.
- 调节性T (Treg) 和B (Breg) 细胞对于免疫平衡和控制高炎症至关重要.
研究的目的:
- 为了研究与COVID-19严重程度相关的Treg和Breg细胞水平的变化.
- 探索Treg/Breg细胞水平,对I型干扰素 (IFN) 的自身抗体和疾病严重程度之间的关联.
主要方法:
- 来自62名COVID-19患者的血液样本的流细胞计分析,疾病严重程度各不相同.
- 常规 (cTreg) 和非常规 (uTreg) Treg的量化,包括LAG3+亚型.
- 评估IL-35+和IL-10+布雷格细胞比例.
主要成果:
- 严重的COVID-19病例显示,与较轻的病例相比,cTreg,uTreg和LAG3+ Treg的比例增加.
- 在患有I型IFN自身抗体的严重病例中,升高的cTreg/uTreg水平更为明显.
- 在严重的COVID-19患者中,IL-35+和IL-10+Breg比例更高,特别是那些对I型IFN具有自身抗体的人.
- 调节性细胞水平的增加与较低的促炎性细胞因子 (IL-6,IL-17,TNFα,IL-1β) 相相关.
- 用托西利祖马布和/或高剂量维生素D治疗的患者表现出更高的调节细胞水平和更好的细胞因子控制.
结论:
- COVID-19与免疫调节Treg和Breg细胞种群的显著干扰有关.
- 对I型IFN的自身抗体的存在加剧了这些Treg/Breg细胞变异在严重的COVID-19中.
- 恢复Treg/Breg细胞平衡可能是管理严重COVID-19炎症的治疗策略.
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