基于它们的形态学和原生学,对三核菌 AG-E 的表征
Ewa Moliszewska1, Dagna Maculewicz2, Hanna Stępniewska3
1Faculty of Natural Sciences and Technology, Institute of Environmental Engineering and Biotechnology, University of Opole, Opole, Poland. ewamoli@uni.opole.pl.
Scientific reports
|October 13, 2023
概括
菌类的分类是通过核数来精炼的. 一个特定的AG-E分离物组始终显示每个细胞有三个细胞核,有助于它们的识别.
科学领域:
- 菌类学 菌类学是指菌类学.
- 植物病理学 植物病理学
背景情况:
- 根據核的數量,Rhizoctonia屬被廣泛分為雙核和多核菌株.
- 准确的分类对于理解Rhizoctonia物种的生物学和病原性至关重要.
研究的目的:
- 为了研究AG-E组内的Rhizoctonia分离物的核特性.
- 确定核数是否可以帮助初步识别Rhizoctonia AG-E分离物.
主要方法:
- 进行DNA测序 (ITS1-5,8S-ITS2rDNA) 进行解组 (AG) 分类.
- 用测试分离物进行解剖反应以确认AG附属性.
- DAPI染色和光显微镜用于计算每个细胞的细胞核.
- 测量形直径的方法.
主要成果:
- 鉴定出12个波兰树枝菌孤立物属于AG-E.
- 并非所有AG-E分离物都表现出均的核数;一个子组始终显示出平均3个核的细胞 (范围为2-4).
- 这些三核酸分离物被指定为AG-E-3n,以促进它们的初步识别.
结论:
- 核数,特别是三个核的存在,对于初步识别Rhizoctonia AG-E分离物来说是一个有价值的特征.
- 胺需求不是对AG-E菌株进行分类的可靠因素.
- 鉴定AG-E-3n分离物质简化了Rhizoctonia物种的分化.
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