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Laura Corneillie¹, Irma Lemmens², Claire Montpellier³

¹Laboratory of Liver Infectious Diseases (LLID), Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, Building MRBII, Corneel Heymanslaan 10, 9000, Ghent, Belgium. laura.corneillie@ugent.be.

Cellular and molecular life sciences : CMLS

|October 13, 2023

概括

肝炎E病毒 (HEV) 使用T细胞免疫球蛋白和粘素域含蛋白1 (TIM1) 进入细胞. 这种由脂胺在包裹的HEV上调解的相互作用对于病毒传播至关重要.

科学领域:

病毒学病毒学
细胞生物学细胞生物学
免疫学免疫学免疫学

背景情况:

肝炎E病毒 (HEV) 仍然不太了解,对其生命周期,致病性和缺乏特定抗病毒药物的知识有限.
HEV存在于非包裹和准包裹的形式,具有不同的细胞进入机制.
包裹式HEV利用氨酸氨酸 (PS) 进行"亡模仿",劫持宿主细胞的感染过程.

研究的目的:

调查T细胞免疫球蛋白和粘素域含蛋白1 (TIM1) 在肝炎E病毒的细胞进入中的作用.
阐明 HEV 进入宿主细胞的机制,重点关注与 TIM1.1 的相互作用.

主要方法:

在有或没有TIM1 (淘汰赛和宫外表达) 的细胞中对HEV感染进行测定.
病毒结合测试以评估HEV粒子的附着性.
分析TIM1在PS结合口袋中的突变对HEV感染的影响.
评估Annexin V,一种PS结合分子,对HEV传染性的评估.

主要成果:

通过包裹颗粒 (eHEV) 的HEV感染在TIM1-Knockout细胞中显著受损.
通过TIM1的表达恢复了eHEV感染,并通过宫外TIM1的表达增强了eHEV感染.
病毒结合和感染因TIM1的PS结合口袋和Annexin V的突变而减少,证实了PS-TIM1相互作用的作用.

结论:

TIM1在包裹式肝炎E病毒 (eHEV) 进入细胞中发挥着重要作用.
病毒膜上的PS与TIM1之间的相互作用促进了eHEV的进入.
这种PS-TIM1相互作用代表了HEV促进系统性病毒传播的潜在策略.

关键词:

阿波托斯的模仿行为.细胞表面受体细胞表面受体这就是HAVCR1的原因.肝炎E病毒的感染.含有T细胞免疫球蛋白和粘素域蛋白1的T细胞免疫球蛋白.病毒的入口病毒的入口病毒性肝炎的病毒性肝炎.病毒与主机的互动

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