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基因组被侵蚀的Burkholderia共生体的营养补充,是状昆虫的共生体

Anna Michalik¹, Eugen Bauer², Teresa Szklarzewicz³

¹Department of Developmental Biology and Morphology of Invertebrates, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University, Krakow, Poland. a.michalik@uj.edu.pl.

The ISME journal

|October 13, 2023

概括

规模昆虫宿主Burkholderia细菌具有高度减少的基因组,提供必要的营养素. 这种共生,虽然年轻,显示功能融合,并突出Burkholderia.

科学领域:

微生物学和进化生物学
昆虫-微生物共生关系
基因组学就是基因组学.

背景情况:

半类动物依靠细菌或真菌共生体获得必需的营养素,因为它们的特殊饮食.
虫 (Coccomorpha) 在他们的共生关系中表现出了显著的多样性.
研究人员研究了Eriococcidae家族的状昆虫Acanthococcus aceris和Gossyparia spuria,寻找它们的共生体.

研究的目的:

在功能上描述Acanthococcus aceris和Gossyparia spuria的细菌共生体.
分析这些虫共生体的基因组特征和代谢能力.
为了推断Burkholderia-eriococcid共生的进化年龄和功能融合.

主要方法:

显微镜检查以确定宿主组织内的共生体定位.
超基因组测序来分析共生细菌的基因组.
对营养素和辅助因子的保留生物合成途径的生物信息分析.

主要成果:

鉴定出Burkholderia细菌是共生生物,它们位于两种虫的脂肪体细胞中.
这些共生生物具有高度减少的AT偏差基因组 (<900Kbp,~38%GC),这是迄今为止测序的最小的Burkholderia基因组.
关键的生物合成途径对于必需的氨基酸 (氨酸,单氨酸,氨酸,氨酸,氨酸,氨酸,氨酸,氨酸,氨酸,氨酸),氨酸前体,可巴拉胺依赖的氨酸合成酶 (MetH) 和多个辅助因子 (利博氨酸,可巴拉胺,氨酸,叶酸) 被保留.

结论:

伯克霍尔迪亚和埃里奥科克斯之间的共生似乎在进化上比其他半球体共生更年轻,但表现出功能上的融合.
托生物合成途径的差异性保存表明,宿主物种之间的食托可用性不同.
Burkholderia被证实是多功能共生体,能够在不同的宿主中进行细胞内和细胞外协会,包括动物,植物和真菌.

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