用Corynebacterium glutamicum进行植物天然产品前体p-酸的微生物合成
Mario Mutz1,2, Dominic Kösters1,2, Benedikt Wynands1
1Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich, 52425, Jülich, Germany.
Microbial cell factories
|October 14, 2023
概括
微生物的p-coumaric 酸的生产是在工程Corynebacterium glutamicum*中实现的. 这项研究优化了石基胺途径和异质基因表达,以有效地从葡萄糖中合成这种有价值的植物多原先体.
科学领域:
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
- 微生物生物技术 微生物生物技术
背景情况:
- 烯胺,像p-酸,是药理学上相关的植物二次代谢物的重要前体.
- 微生物合成为植物提取或化学合成提供了一种具有成本效益和资源效率的替代方案.
- 这项研究的重点是工程 *Corynebacterium glutamicum* 基于葡萄糖的p-酸生产.
研究的目的:
- 为了工程 *Corynebacterium glutamicum* 从葡萄糖中有效的微生物生产p-coumaric酸.
- 为了优化代谢途径,以增强前体积累.
- 为了证明下游代谢物合成的共同培养潜力.
主要方法:
- 来自*Flavobacterium johnsoniae*的铁氨酶的异质表达.
- 消除烯酸的降解途径,并通过突变发生来减少烯酸副产品的形成.
- 增强碳流入石基酸盐通路并改善基酸的可用性.
- 减少氨生物合成并利用反耐药的酶变体.
主要成果:
- 在定义的矿物介质中达到661 mg/L (4 mM) p-酸的最大标位.
- 通过针对性转基因转基因合成酶的向突变,成功消除了甲酸盐的积累.
- 从葡萄糖中直接合成31.2 mg/L (0.14 mM) 的复星.
结论:
- 不同类型的氨酸氨基酶和石基胺路径的优化是*C. glutamicum*中高效的p-酸生产的关键.
- 从 fenilalanine 和 tryptophan 路径重定向碳流对于高产量生产至关重要.
- 工程*C. glutamicum*为生产有价值的烯胺提供了一个坚固的平台.
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