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相关概念视频

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Genome Annotation and Assembly03:36

Genome Annotation and Assembly

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
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Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
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RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

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Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Protein Complex Assembly02:41

Protein Complex Assembly

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相关实验视频

Updated: Jul 13, 2025

Identification of Circular RNAs using RNA Sequencing
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JCcirc:通过集成的结接连接通过circRNA全长序列组合.

Jingjing Zhang1,2, Huiling Zhang3, Zhen Ju1,2

  • 1University of Chinese Academy of Sciences, Beijing, China.

Briefings in bioinformatics
|October 14, 2023
PubMed
概括

一种新的方法,JCcirc,准确地组装了全长的圆形RNA (circRNA) 序列及其异形. 该工具改进了现有方法,使得用于疾病诊断和疫苗开发的circRNAs的功能分析更好.

关键词:
环RNA 环RNA 是一个环RNA.一个全长的序列.交叉点的连接 连接转录组 (transcriptome) 是一个转录组.

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相关实验视频

Last Updated: Jul 13, 2025

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科学领域:

  • 生物信息学是一种生物信息学.
  • 基因组学就是基因组学.
  • 分子生物学分子生物学

背景情况:

  • 循环RNAs (circRNAs) 在疾病生物标志物和核酸疫苗中表现有前途.
  • 准确的全长circRNA序列组装对于探索这些应用至关重要.
  • 目前的组装工具在正确组装所有circRNAs方面存在局限性.

研究的目的:

  • 开发一种用于高效和准确的全长circRNA序列重建的新方法.
  • 改进所有circRNA类型及其替代异型的组装.
  • 为 circRNA 功能分析提供一个强大的工具.

主要方法:

  • 引入连接连接 (JC) 功能,延长后联接连接 (BSJ).
  • 开发JCcirc,一种利用JC和BSJ的新方法.
  • 拼接图和碎片覆盖的应用,用于circRNA组装.

主要成果:

  • 在人体模拟数据集上,JCcirc表现出比现有方法更优异的性能,F1得分明显更高.
  • 该方法显示了在各种circRNA大小范围内改善的正确组装速率.
  • 此外,JCcirc对其他物种的数据集和真实测序数据的性能优于现有的工具.

结论:

  • JCcirc 是一个强大而准确的工具,用于组装全长 circRNA 序列和替代异构.
  • 这一进步有助于对circRNAs进行更深入的功能分析.
  • 这些发现为未来基于circRNA的诊断和治疗奠定了基础.