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确定高强度脉冲电磁场对释放与损伤相关的分子模式分子的影响

Matej Kranjc¹, Tamara Polajžer¹, Vitalij Novickij^2,3

¹Faculty of Electrical Engineering, University of Ljubljana, Trzaska cesta 25, 1000 Ljubljana, Slovenia.

International journal of molecular sciences

|October 14, 2023

概括

高强度脉冲电磁场 (HI-PEMF) 治疗通过导致损伤相关分子模式 (DAMP) 的有限释放,最小地激活了免疫反应. 这种非侵入性方法显示了基因转移和电化学疗法的潜力,减少了免疫细胞透.

科学领域:

生物医学工程生物医学工程
细胞生物学细胞生物学
免疫学免疫学免疫学

背景情况:

高强度脉冲电磁场 (HI-PEMF) 为基因转移和电化学疗法提供了电穿孔的非侵入性替代方案.
高-PEMF远程诱导电场,可能最大限度地减少免疫细胞透.
电化疗中的免疫反应激活可能与免疫细胞死亡和损伤相关分子模式 (DAMPs) 有关.

研究的目的:

在试管体内研究HI-PEMF治疗后的DAMP (ATP,calreticulin,HMGB1) 的释放.
为了比较HI-PEMF后的DAMP释放与常规电穿孔.
评估HI-PEMF治疗的潜在免疫反应激活.

主要方法:

在体外研究使用三个不同的细胞系.
使用高强度脉冲电磁场 (HI-PEMF) 进行治疗.
与传统的电穿孔参数进行比较.
测量释放的DAMP分子:腺三酸盐 (ATP),卡莱蒂库林和高流动性组盒1蛋白 (HMBG1).

主要成果:

治疗HI-PEMF并没有诱导HMGB1或calreticulin的释放.
在HI-PEMF治疗后立即检测到腺三酸盐 (ATP) 释放.
总的来说,HI-PEMF治疗导致DAMP分子的释放不到最小.

结论:

HI-PEMF治疗导致DAMP分子的释放最小.
有限的DAMP释放表明免疫反应的激活最小.
HI-PEMF为治疗应用提供了一种有前途的非侵入性方法,具有降低的免疫性.

关键词:

这就是ATPATPATP ATP.垃圾填埋场是一个垃圾填埋场.这就是HI-PEMF.这就是HMGB1的特点.卡尔雷蒂库林是一种卡尔雷蒂库林.电磁场是一种电磁场.免疫反应的免疫反应.

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