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植物性大麻素降低了原始巨细胞和肠道质细胞的炎症:一个体外研究

Gal Cohen¹, Ofer Gover¹, Betty Schwartz¹

¹Department of Biochemistry Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Herzl Street 229, Rehovot 7610001, Israel.

International journal of molecular sciences

|October 14, 2023

概括

四大麻素 (THC) 通过减少炎症标志物,在肠道细胞中显示出抗炎作用. 这项研究研究了植物性大麻素对炎症性肠病 (IBD) 治疗潜力的研究.

科学领域:

胃肠病学胃肠病学
免疫学免疫学免疫学
药理学药理学是指药理学的学科.

背景情况:

肠道炎症涉及肠道质细胞 (EGC) 和巨细胞.
植物性大麻素可以通过调节内分泌系统来治疗炎症性肠病 (IBD).
内分泌系统存在于免疫和肠道细胞中.

研究的目的:

研究九种纯大麻素对巨细胞和EGCs的抗炎作用.
评估大麻素对炎症基因和蛋白质表达的影响.
分析用THC和THC-COOH处理的EGC中的转录基因变化和受影响的途径.

主要方法:

在体外研究中使用J774A1巨细胞和用脂多糖 (LPS) 刺激的EGC.
在巨细胞中测量TNFα (瘤亡因子-α) 和EGC中的S100B/GFAP.
RNA-sequencing (RNA-seq) 和Ingenuity途径分析,以确定差异表达的基因和受影响的生物途径.

主要成果:

在较低度下,THC在两种细胞类型中表现出最显著的抗炎作用.
RNA-seq分析揭示了用LPS,THC或THC-COOH治疗的EGC中的差异性基因表达.
THC影响了mTOR和RAR信号通路,而THC-COOH主要影响了IL-6信号通路.

结论:

在肠道巨细胞和EGC中,THC表现出强大的抗炎性质.
THC及其代谢物THC-COOH调节与炎症相关的独特信号通路.
这些发现支持植物大麻素在控制肠道炎症和IBD方面的潜在治疗作用.

关键词:

J774A1 M1 巨细胞中的一个.肠道质细胞肠道质细胞植物大麻素 (phytocannabinoids) 是一种植物性大麻素.

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