通过暴露于极低频脉冲电磁场来减少NET形成
Caren Linnemann1, Filiz Sahin1, Yangmengfan Chen1
1Siegfried Weller Institute for Trauma Research, BG Unfallklinik Tübingen, Eberhard Karls Universität Tuebingen, Schnarrenbergstraße 95, 72076 Tuebingen, Germany.
International journal of molecular sciences
|October 14, 2023
概括
极低频脉冲电磁场 (ELF-PEMF) 疗法不会刺激中性粒细胞形成中性粒细胞外细胞陷 (NET). 这一发现支持立即使用ELF-PEMF用于骨折愈合,因为它不会阻碍愈合过程.
科学领域:
- 生物医学工程 生物医学工程
- 细胞生物学 细胞生物学
- 整形外科 整形外科 整形外科
背景情况:
- 骨折愈合是复杂的,而不能愈合的骨折会带来重大的临床挑战.
- 极低频脉冲电磁场 (ELF-PEMF) 正被探索为骨愈合的非侵入性辅助疗法.
- 人们担心ELF-PEMF诱导的活性氧物种 (ROS) 和流入 (Ca2+) 可能过度刺激中性粒细胞,导致中性粒细胞细胞外陷 (NET) 形成,可能阻碍愈合.
研究的目的:
- 研究16赫兹ELF-PEMF对中性粒细胞外细胞陷 (NET) 形成的影响.
- 确定ELF-PEMF是否会诱导中性粒细胞中的ROS和Ca2+流入,这些都是已知的NET刺激.
主要方法:
- 从健康的志愿者中分离出中性粒细胞.
- 细胞被暴露在16赫兹ELF-PEMF和各种NET诱导刺激 (PMA,LPS,H2O2) 中.
- 使用赛托克斯绿色测试和免疫光学量化了NET形成;用光探针测量了Ca2+流入和ROS.
主要成果:
- 与介质细胞不同,ELF-PEMF没有诱导中性粒细胞中的ROS或Ca2+流入.
- 暴露于ELF-PEMF并没有增加基底或PMA刺激的NET形成.
- ELF-PEMF显著减少了由PMA,LPS和H2O2.2诱导的NET中的DNA释放.
结论:
- 16赫兹ELF-PEMF不会诱导NET形成或对中性粒细胞产生负面影响.
- 这些发现表明,ELF-PEMF可以在骨折治疗后立即安全地启动,而不会影响愈合级联.
- ELF-PEMF显示出作为一种安全有效的辅助疗法来增强骨折愈合的潜力.
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