基于NMR的酵母Oxa1-CTD和核糖体之间的相互作用的表征
Yong Liu1,2, Jing Yang1, Maosen Ruan1
1High Magnetic Field Laboratory, Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China.
International journal of molecular sciences
|October 14, 2023
概括
在蛋白质合成过程中,线粒体Oxa1 C端 (Oxa1-CTD) 与线粒体核糖体相互作用. 这种以特定的接触点和结构变化为特征的相互作用对于组装氧化酸化复合体至关重要.
科学领域:
- 线粒体生物学 线粒体生物学
- 蛋白质的生物发生.
- 结构生物学是结构生物学.
背景情况:
- 线粒体核糖体合成关键的氧化酸化 (OXPHOS) 子单元.
- 线粒体氧化酶组合蛋白1 (Oxa1) 促进了这些子单元的同翻译性插入到线粒体内膜 (IMM).
- Oxa1 (Oxa1-CTD) 的C端参与了与核糖体的这一关键相互作用.
研究的目的:
- 为了结构性地描述酵母酵母Oxa1-CTD.
- 为了阐明Oxa1-CTD与70S核糖体之间的相互作用模式.
- 了解Oxa1-CTD在线粒体蛋白组装中的作用.
主要方法:
- 核磁共振 (NMR) 光谱法用于结构性研究.
- 使用酵母Oxa1-CTD和*E. coli*70S核糖体进行了相互作用研究.
主要成果:
- 在核糖体结合后,Oxa1-CTD采用一种短暂的α-螺旋结构 (残留物P342-Q385).
- 确定了Oxa1-CTD (N端V321-I327和内部I348-Q370) 上两个保存的接触点,用于核糖体相互作用.
- 一个模型表明,Oxa1-CTD相互作用涉及第二个接触点的短暂到稳定的结构过渡.
结论:
- 在核糖体相互作用期间,Oxa1-CTD表现出特定的结构动态.
- 鉴定到的接触点和结构过渡提供了对Oxa1介导的共同翻译插入的机制性见解.
- 这些发现提高了对OXPHOS复合组件和Oxa1-CTD功能的理解.
更多相关视频
09:04Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis
Published on: July 26, 2018
7.8K
05:44Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes
Published on: November 9, 2020
3.6K
相关概念视频
02:24Ribosome Profiling
3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
01:44Termination of Translation
25.4K
The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...
25.4K
01:56Riboswitches
8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K