化学因子与田素-C结合 影响化学因子诱导的免疫细胞迁移
Alissa Domaingo1, Philipp Jokesch1, Alexandra Schweiger1
1Institute of Pharmaceutical Sciences, Karl-Franzens-University Graz, Schubertstr. 1, 8010 Graz, Austria.
International journal of molecular sciences
|October 14, 2023
概括
素-C (TNC) 通过差异调节免疫细胞迁移. 它通过CCL26增强了乙氨基基细胞化学反应,但减少了CCL2介导的单细胞迁移,影响了炎症反应.
科学领域:
- 生物化学 生物化学
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 素-C (TNC) 是一种细胞外基质糖蛋白,涉及瘤发生和炎症.
- 化基因是这些病理过程中的关键调解者.
- 了解TNC与化学因子的相互作用对于破译免疫细胞调节至关重要.
研究的目的:
- 为了研究关键化学因子 (CCL2,CCL26,CXCL8,CXCL10,CXCL12) 与特纳辛-C (TNC) 的结合模式.
- 确定TNC对化学因子介导的免疫细胞化学反应的影响,特别是乙酸细胞和单细胞.
- 在细胞外矩阵相互作用的背景下阐明TNC在调节免疫细胞迁移中的作用.
主要方法:
- 化学基因结合测试以量化与TNC的亲和力.
- 测量甲酸 (HS) 与TNC的结合亲和力.
- 免疫细胞动员实验 (eosinophils和单细胞) 有或没有TNC预化作为对特定化学因子的反应.
主要成果:
- 在TNC和测试的化学激素之间观察到不同的结合亲和力,CCL26显示高亲和力,CCL2显示低亲和力.
- 肝硫酸盐 (HS) 证明了与TNC的高亲和度结合 (Kd = 730 nM).
- TNC显著增加了CCL26诱导的乙氨基基细胞化学反应 (3.5倍),但降低了CCL2诱导的单细胞化学反应 (1.3倍).
结论:
- 对于不同的化基因,TNC表现出明显的结合亲和力.
- TNC调节免疫细胞化学反应,作为一个放大剂对乙酸细胞迁移 (CCL26) 和一个减弱剂对单细胞迁移 (CCL2).
- 这些发现表明,TNC在免疫细胞贩运中发挥着调节作用,通过影响细胞外基质内的化学基因-海帕兰硫酸盐相互作用.
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