表观遗传标记作为对腹手术代谢反应的预测:从表观基因组广泛协会研究中的验证
Carolina Gutiérrez-Repiso1,2,3, Antonio Cantarero-Cuenca4, Andrés González-Jiménez4
1Unidad de Gestión Clínica de Endocrinología y Nutrición, Hospital Clínico Universitario Virgen de la Victoria, Campus de Teatinos s/n, 29010 Málaga, Spain.
International journal of molecular sciences
|October 14, 2023
概括
表观遗传标记,特别是像CYP2E1这样的基因中的DNA甲基化模式,可以预测减肥手术后的代谢改善. 这些甲基化变化可以作为治疗反应的生物标志物.
科学领域:
- 表观遗传学和代谢健康
- 腹部外科手术的结果
- 基因组生物标志物 基因组生物标志物
背景情况:
- 与肥胖相关的并发症显著影响患者的健康.
- 减肥手术是有效的,但预测个体反应仍然具有挑战性.
- 表观遗传修饰,如DNA甲基化,与代谢调节有关.
研究的目的:
- 调查DNA甲基化的作用作为代谢性并发症解决的预测因素,在腹腔外科手术后.
- 为了确定与袖子胃切除术后成功的代谢改善相关的特定表观遗传标记.
主要方法:
- 分析了经过袖子胃切除术的20名患者的DNA甲基化,根据6个月的代谢综合征解决方案将他们分类为反应者或不反应者.
- 使用Infinium甲基化EPIC珠芯片阵列评估26个CpG站点的甲基化水平.
- 专注于CpG位点,对以前与代谢状态相关的基因进行注释,包括CYP2E1,HLA-DRB1和ZFPM2.2.
主要成果:
- 细胞染色体P450 2E1 (CYP2E1) 基因 (cg11445109,cg19469447) 中的特定CpG位点在响应者中与不响应者相比显著低甲基化.
- 核蛋白与MKI67伪基因3 (NIFKP3) 基因 (cg25828445) 的FHA域相互作用的核蛋白中的一个CpG位点在非响应者中显著高甲基化.
- CYP2E1,HLA-DRB1和ZFPM2基因显示出差异甲基化CpG位点的比例最高.
结论:
- 特定CpG位点的DNA甲基化模式,特别是与CYP2E1相关的DNA甲基化模式,可以作为对减肥手术代谢反应的预测生物标志物.
- 这些发现突显了表观遗传分析的潜力,以个性化减肥手术结果和患者管理.
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