在下下垂体出血患者中循环细胞外囊泡:特征和细胞效应
Elena Grossini1, Teresa Esposito2,3, Michela Viretto2,3
1Laboratory of Physiology, Department of Translational Medicine, Università del Piemonte Orientale, 28100 Novara, Italy.
International journal of molecular sciences
|October 14, 2023
概括
副arachnoid出血 (SAH) 患者的细胞外囊泡 (EV) 通过破坏内皮细胞和增加光滑肌肉反应性,有助于血管 (VP). 这些改变的EV有潜力成为SAH并发症的诊断标志物.
科学领域:
- 心血管研究研究心血管研究
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
背景情况:
- 甲状腺下出血 (SAH) 可以导致严重的并发症,如血管 (VP).
- 细胞外囊泡 (EVs) 越来越多地被认为是它们在各种病理生理过程中的作用.
- 循环EVs对SAH病原发生的具体贡献,特别是VP,仍然不完全理解.
研究的目的:
- 描述SAH患者的循环EV,区分具有和没有VP的患者.
- 研究SAH衍生的EVs对人类血管内皮细胞 (HUVEC) 和光滑肌细胞 (SMC) 的功能影响.
主要方法:
- 从18名SAH患者 (10名有VP,8名没有VP) 和5名健康对照 (HC) 中分离出血EV.
- 电动汽车的特点是表面标记,尺寸和度.
- 孤立的EV被用于激发HUVEC和SMCs在体外以评估细胞反应.
主要成果:
- 从SAH衍生的EV显示T淋巴细胞和血小板标记物,与HC相比,其大小和度增加.
- 来自VP患者的EVs降低了HUVEC活力和线粒体功能,增加了氧化剂和氧化 (NO) 的释放.
- 来自SAH的EV在SMC中的细胞内水平升高,表明反应性增强.
结论:
- 在SAH患者中,循环EVs发生变化,这表明在内皮功能障碍和SMC过度反应性中具有病原性作用.
- 这些发现凸显了EVs作为SAH并发症的诊断和预后生物标志物的潜力.
- 电动血管可能是治疗SAH诱导血管的新治疗标.
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