验证SARS-CoV-2替代中和测试检测中和抗体对主要变种的关注
Eveline Santos da Silva1, Jean-Yves Servais1, Michel Kohnen2
1Department of Infection and Immunity, Luxembourg Institute of Health, 29 Rue Henri Koch, L-4354 Esch-sur-Alzette, Luxembourg.
International journal of molecular sciences
|October 14, 2023
概括
一种适应新变异的代理病毒中和测试 (sVNT) 准确地预测了对SARS-CoV-2关注变异 (VOC) 的免疫保护. 这项测试与既有方法有很强的相关性,有助于评估疫苗的有效性.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 传染性疾病 传染性疾病
背景情况:
- 感染SARS-CoV-2和接种疫苗会诱导对关注变种 (VOC) 的中和抗体.
- 准确评估中和活性对于了解免疫保护至关重要.
- 现有的方法可能需要对新出现的SARS-CoV-2变种进行进一步验证.
研究的目的:
- 建立和验证一种适应变异的代理病毒中和试验 (sVNT).
- 评估对祖先的SARS-CoV-2 (WT) 和VOCs (Delta,Omicron BA.1,BA.2,BA.5) 的中和活性.
- 将sVNT的分析性能与参考病毒中和测试 (VNT) 和商业套件进行比较.
主要方法:
- 开发一种适应变异的代理病毒中和试验 (sVNT).
- 测试对WT和VOC的中和活性 (Delta,BA.1,BA.2,BA.5).
- 用参考VNT和两个CE-IVD标记套件进行对比分析,跨越三个COVID-19波浪队列.
主要成果:
- sVNT与WT,Delta,BA.1,BA.2和BA.5.5的VNT显示了中度至强度的相关性.
- 与商业套件的性能比较显示出良好的相关性,对WT-sVNT具有87.76%的灵敏度和90.48%的临床特异性.
- BA.2-sVNT的性能与商业BA.2测试相当,而BA.5-sVNT与VNT.显示出强烈的关联.
结论:
- 开发的适应变异的sVNT有效地预测了对SARS-CoV-2 VOCs的免疫保护.
- sVNT提供了一种可靠的替代方法,用于评估针对各种病毒菌株的中和活性.
- 该工具支持对疫苗有效性和免疫反应对演变的SARS-CoV-2变体的评估.
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