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通过双子病毒的短暂表达,快速高效地合成胡中的桑

Zhimin Lin¹, Muhammad Moaaz Ali², Xiaoyan Yi²

¹Fujian Academy of Agricultural Sciences Biotechnology Institute, Fuzhou 350003, China.

International journal of molecular sciences

|October 14, 2023

概括

这项研究通过使用双子病毒来提高桑丁的产量,增强了果的红色. 这种方法迅速增加了桑的含量,改善了消费者的的质量.

科学领域:

植物生物技术植物生物技术
代谢工程是代谢工程.
胡卜素生物合成胡卜素生物合成

背景情况:

果的颜色,由桑积累决定,对于消费者偏好至关重要.
素的产生是由素/素合成酶 (CCS) 和胡卜素基酶 (CrtZ) 的基因表达调节的.

研究的目的:

通过使用双胞胎病毒技术,研究中桑丁合成的增强.
探索异质基因表达对内源基因转录和代谢物积累的影响.

主要方法:

利用双子病毒 (豆黄矮人病毒) 在植物中的基因表达.
采用了编码子优化异质β-胡卜素氧酶 (CrtZ) 和β-胡卜素酶 (CrtW) 的表达.
监测了CCS和内源CrtZ基因的转录水平.

主要成果:

双子病毒介导的CrtZ和CrtW的表达显著增加了桑的积累.
异质CrtZ和CrtW的增强表达导致CCS基因和内源CrtZ的转录增加.
在短时间内实现了快速和大量的桑积.

结论:

基于双子病毒的表达提供了一个新的平台,可以快速增强植物中的桑生产.
这种方法提供了一种通过加速胡卜素生物合成来改善的质量策略.
这项研究证明了双子病毒在农业应用中的代谢工程方面的潜力.

关键词:

卡普桑提因 (capsanthin) 是一种含有卡普桑提因的物质.胡卜素是一种胡卜素.双胞胎病毒双胞胎病毒胡胡胡胡是一个过渡性系统的过渡性系统

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