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可追溯性关于Dendrobium devonianum的研究基于SWATH到MRM

Tao Lin¹, Xinglian Chen¹, Lijuan Du¹

¹Quality Standards and Testing Technology Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China.

Foods (Basel, Switzerland)

|October 14, 2023

概括

通过分析Dendrobium devonianum和Dendrobium officinale之间的化学差异,SWATHtoMRM技术有效地追溯了Dendrobium的起源. 这种伪向的代谢学方法确定了特征性化合物和代谢途径,有助于品种改进.

关键词:

丹德罗比亚姆 (Dendrobium devonianum) 是一种植物.龙陵地区的长林地区.标识标识标识标识标识飞行时间质谱测量

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科学领域:

代谢学代谢学代谢学
分析化学分析化学
药理学是指药理学,它是指药理学.

背景情况:

区分密切相关的药用植物,如Dendrobium devonianum和Dendrobium officinale对于质量控制和可追溯性至关重要.
传统方法往往难以区分具有相似起源和外观的物种.
需要先进的分析技术来发现微妙的生化差异.

研究的目的:

开发和应用一种伪准代谢学方法,用于追踪和识别Dendrobium devonianum和Dendrobium officinale.
为了研究这两个Dendrobium物种的独特化学成分.
为了确定特征性化合物和丰富的代谢途径,以潜在地用于品种改进.

主要方法:

使用SWATHtoMRM技术与飞行时间 (TOF) 和多重反应监测 (MS-MRM) 质谱相结合.
使用R包处理数据以获得MRM对 (191个正,96个负离子).
应用主要组件分析 (PCA) 和部分最小平方区分分析 (PLS-DA) 用于数据分析和物种差异化.

主要成果:

根据它们的MRM离子配置文件,成功分离了Dendrobium devonianum和Dendrobium officinale,这表明化学组成有显著差异.
确定了146种特征性化合物,其中20种是唯一的Dendrobium devonianum.
丰富分析揭示了关键途径,包括脂质和动脉样硬化,脂信号传递和氨酸/氨酸代谢,11种化合物影响炎症标志物.

结论:

基于SWATHtoMRM的伪向代谢学方法对于Dendrobium物种的可追溯性和识别是有效的.
在Dendrobium devonianum和Dendrobium officinale之间存在显著的代谢差异,提供潜在的生物标志物.
鉴定的特征性化合物和途径为Dendrobium繁殖和活性物质的积累提供了宝贵的见解.