在乳腺上皮细胞中TAZ结合部位的全基因组特征
Tao Liu1, Jiaojiao Zhou1, Yanmin Chen2
1Department of Biostatistics and Bioinformatics, Roswell Park Comprehensive Cancer Center, Elm Street, Buffalo, NY 14203, USA.
Cancers
|October 14, 2023
概括
具有PDZ结合动机 (TAZ) 的转录共激活剂驱动基底样乳腺癌的进展. 这项研究绘制了TAZTA的地图.
科学领域:
- 分子生物学分子生物学
- 癌症研究 癌症研究
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 具有PDZ结合动机 (TAZ) 的转录共激活剂是Hippo信号通路的关键效应因子.
- 高TAZ表达与基础性乳腺癌 (BLBC) 的生存率和复发差相关.
- TAZ的致癌作用与通过不太了解的基因程序调节增殖,迁移和亡抵抗有关.
研究的目的:
- 在乳腺上皮细胞中以TAZ调节的转录程序进行时间和空间的表征.
- 在TAZ激活后识别全球TAZ绑定站点.
- 阐明TAZ在调节促进乳腺癌发展的基因表达程序中的机制.
主要方法:
- 在MCF10A细胞中使用了一种可引系统.
- 使用RNA测序来进行TAZ调控转录的时间和空间特征.
- 进行了ChIP测序和ATAC测序,以确定TAZ结合部位和染色质变化.
主要成果:
- 在TAZ被激活后,TAZ迅速本地化以增强区域,后来扩展到促进区域.
- TAZ与增强剂的结合涉及TEAD和FOSL2转录因子动机的切换.
- TAZ激活诱导了显著的染色质结构变化.
结论:
- 这项研究揭示了TAZ结合部位的全基因组景观.
- 研究结果提供了关于TAZ如何调节乳腺癌中的基因表达程序的见解.
- 这些结果可能会增强对TAZ对乳腺癌发展的贡献的理解.
关键词:
ATAC-seqq 的使用情况.在 ChIP-seqq.这就是FOSL2的原因.塔兹 (TAZ) 是一个名为塔兹 (TAZ) 的城市.我们的 TEAD TEAD 团队.乳腺癌 乳腺癌 乳腺癌增强剂是一种增强剂.更多相关视频
11:31Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands
Published on: May 30, 2017
10.1K
06:54Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
Published on: October 27, 2020
13.0K
相关概念视频
Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Tail-anchoring of Proteins in the ER Membrane
Tail-anchored, or TA, proteins are estimated to make up to 3-5% of membrane proteins found in the eukaryotic cell. Such proteins have a single transmembrane domain located approximately 30 amino acid residues upstream from the C-terminal end. As a result, the signal recognition particle (SRP) cannot guide a TA protein to the ER membrane for cotranslational insertion. Hence, they are integrated into the ER membrane post-translationally using their C-terminal end as the anchor. TA proteins...
General Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase
Genetic polymorphisms in drug targets have emerged as critical determinants of interindividual variability in drug response and toxicity. Pharmacogenomic investigations increasingly focus on identifying these variations to personalize and optimize therapeutic interventions. A drug target may be a receptor, enzyme, or signaling protein involved in pharmacologic responses or disease-related pathways. While early pharmacogenetic studies focused primarily on drug metabolism, current research...
