相关实验视频

Updated: Jul 13, 2025

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

使用等离子纳米探针辅助多模无线光学显微镜同时进行植物细胞的3D构造和成像

Kun Liu¹, Yutian Lei², Dawei Li¹

¹School of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, Dalian 116024, China.

Nanomaterials (Basel, Switzerland)

|October 14, 2023

概括

这项研究引入了一种使用等离子纳米探针和多式非线性光学显微镜进行体内植物细胞成像的新方法. 这种技术可以同时进行细胞结构的3D标签和成像,提供快速和经济有效的解决方案.

科学领域:

植物细胞生物学植物细胞生物学
纳米技术纳米技术
光学显微镜是一种光学显微镜.

背景情况:

非线性光学 (NLO) 成像为植物细胞分析提供了优势,包括深度透和高分辨率.
传统的NLO成像通常需要对特定细胞点进行外源纳米探针.
现有的纳米探针制造和标签方法可能是耗时和复杂的.

研究的目的:

开发一种单步体内方法,同时进行植物细胞结构的3D标记和成像.
使用等离子纳米探针辅助多模式NLO显微镜来增强细胞可视化.
为传统的植物细胞标签技术提供快速,经济高效的替代方案.

主要方法:

采用多式NLO显微镜,结合第二生成 (SHG) 和两光子发光 (TPL).
使用贵金属离子 (银或金) 作为现场纳米探针前体.
应用 femtosecond 激光扫描来诱导等离子体纳米粒子形成和细胞壁标签.

主要成果:

使用SHG和TPL信号实现了完整的土豆细胞结构的同时3D成像.
只有在贵金属离子存在的情况下,才能观察到NLO信号,特别是来自细胞壁的NLO信号.
贵金属离子通过激光在细胞壁沿线被减少到等离子纳米颗粒,使选择性标记和成像成为可能.

结论:

塑纳米探针辅助的NLO显微镜使植物细胞的有效的体内标记和3D成像成为可能.
这种方法绕过了预制纳米探针的需求,简化了这个过程.
该方法在植物细胞结构分析的速度和成本效益方面取得了显著的进步.

关键词:

3D 蜂建筑的3D 蜂建设五秒激光激光器是如何使用的多模式非线性光学成像技术贵金属离子贵金属离子植物细胞植物细胞等离子体纳米结构的结构.

更多相关视频

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Multimodal Optical Imaging Platform for Studying Cellular Metabolism

Published on: June 6, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

相关概念视频

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...