肠球菌 (Enterococcus faecium) HDRsEf1促进全身的Th1反应,并增强对沙门氏菌 (Salmonella) 和类型菌 (Typhimurium) 感染的抵抗力
Jin Zhou1,2, Tingyang Wang1,2, Lele Fan1,2
1State Key Laboratory of Agriculture Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Nutrients
|October 14, 2023
概括
肠球菌菌HDRsEf1增强免疫系统的发育,促进T辅助1细胞的反应,改善对沙门氏菌Typhimurium感染的抵抗力. 这种益生菌显示出作为免疫生物剂的潜力.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 胃肠病学 胃肠病学
背景情况:
- 肠道微生物群在调节宿主免疫系统和影响感染易感性方面发挥着至关重要的作用.
- 了解调节免疫反应的特定微生物菌株对于开发新型治疗策略至关重要.
研究的目的:
- 研究Enterococcus faecium HDRsEf1 (HDRsEf1) 对宿主免疫系统的免疫调节作用.
- 评估HDRsEf1作为一种免疫生物剂的潜力,以增强对细菌感染的抵抗力.
主要方法:
- 向小鼠注射HDRsEf1并评估脏指数和白色肉质区域.
- 对T助手 (Th) 1细胞种群和细胞中细胞因子 (IFN-γ,IL-12) 的流细胞计分析.
- 在体外共培实验中,使用CD4+ T细胞和用HDRsEf1.1.治疗的树突细胞进行了同培实验.
- 挑战HDRsEf1治疗的小鼠感染沙门氏菌 Typhimurium,以评估感染抵抗力和基因表达 (TNFα, iNOS).
主要成果:
- HDRsEf1的使用显著增加了脏指数和脏白肉皮区域,表明免疫系统发育有所改善.
- 在体内和体外观察到Th1细胞和Th1相关细胞因子 (IFN-γ,IL-12p70) 的显著增加.
- HDRsEf1治疗增强了对沙门氏菌Typhimurium感染的抵抗力,刺激了TNFα和iNOS基因的表达.
结论:
- Enterococcus faecium HDRsEf1通过促进全身Th1免疫反应,作为一种有效的免疫生物剂.
- HDRsEf1启动宿主免疫系统,增强巨细胞的激活,并加强对抗细菌病原体的防御,如沙门氏菌Typhimurium.
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