相关实验视频

Updated: Jul 13, 2025

Symptom Assessment of Patients with Allergic Rhinitis Using an Allergen Exposure Chamber

Symptom Assessment of Patients with Allergic Rhinitis Using an Allergen Exposure Chamber

Published on: March 3, 2023

评估血清二胺氧化酶作为对基因素不耐受性的诊断测试

Kristina Arih¹, Nina Đorđević¹, Mitja Košnik^1,2

¹Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia.

|October 14, 2023

概括

胺不耐受性 (HIT) 诊断有助于血清二胺氧化酶 (DAO) 水平,但不应仅仅依赖于这种酶测试. 当与临床评估相结合时,DAO测量提供了额外的诊断价值.

科学领域:

胃肠病学胃肠病学
临床生物化学临床生物化学
免疫学免疫学免疫学

背景情况:

基因组胺不耐受 (HIT) 是由于基因组胺分解受损的结果,通常是由于减少胺氧化酶 (DAO) 活性.
积累的组胺会导致各种不同的临床症状.
血清DAO测量是HIT的常见但有争议的诊断工具.

研究的目的:

评估血清二胺氧化酶 (DAO) 水平在疑似基因组胺不耐症 (HIT) 患者的诊断有效性.
评估DAO水平与HIT临床概率之间的相关性.

主要方法:

对249名疑似HIT患者和50名健康对照者的回顾性分析.
对血清DAO水平的测量.
根据临床标准将患者分为高 (n=41) 和低 (n=208) 概率的HIT组.

主要成果:

患有高概率HIT的患者与低概率HIT (10 U/mL) 和健康对照 (18 U/mL) 相比,DAO中位数显著降低 (8 U/mL).
DAO水平<10 U/mL在区分高概率HIT和低概率HIT方面表现出71%的灵敏度和97%的特异性.
制造商对DAO水平的截止值显示出不同的灵敏度和特异性,取决于对比组.

结论:

当与临床评估相结合时,血清DAO测定是诊断HIT的宝贵补充工具.
诊断HIT不应仅仅依赖于DAO测量;临床判断仍然至关重要.

关键词:

诊断诊断诊断诊断诊断诊断氨酸氧化酶是一种二胺氧化酶.胰岛素不耐受性胰岛素不耐受性血液中的血清是血清.

更多相关视频

Basophil Activation Test for Allergy Diagnosis

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Published on: June 29, 2021

相关概念视频

Serum Laboratory Studies, Stool Test, Breath Test

Serum Laboratory Studies, Stool Test, Breath Test

Gastrointestinal (GI) diagnostic studies are pivotal in confirming, ruling out, diagnosing, or staging various diseases, including cancers. Following diagnosis, allocating time for discussions with the patient and providing informational resources is crucial. Diagnostic assessments of the GI tract often occur in outpatient settings like endoscopy suites or GI labs. Preparation for these tests may include dietary restrictions, fasting, liquid bowel preparations, laxatives, enemas, and the...

Amines to Sulfonamides: The Hinsberg Test

Amines to Sulfonamides: The Hinsberg Test

The Hinsberg test is a method to identify primary, secondary and tertiary amines, named after its pioneer, Oscar Hinsberg. Here, amines are treated with benzenesulfonyl chloride, also known as the Hinsberg reagent, in the presence of an excess of aqueous base, followed by acidification. Based on the nature of the amines, different changes are observed.
Generally, a primary amine reacts with the Hinsberg reagent to produce an N-substituted benzenesulfonamide. The electron-withdrawing...