通过酶辅助从绿色宏藻Ulva fenestrata中提取Ulvan
Ana Malvis Romero1, José Julián Picado Morales1, Leon Klose1
1Institute of Technical Biocatalysis, Hamburg University of Technology, Denickestraße 15, 21073 Hamburg, Germany.
Molecules (Basel, Switzerland)
|October 14, 2023
概括
使用酶和超声波从绿藻中提取Ulvan显示出高产量. 来自Ulva fenestrata的残留生物质也是一个有价值的蛋白质来源.
科学领域:
- 海洋生物技术 海洋生物技术
- 生物聚合物科学 生物聚合物科学
- 绿色化学是一种绿色化学.
背景情况:
- 乌尔万是一种来自绿藻的硫酸多糖,为可持续的生物材料提供生物相容性和生物降解性.
- 它作为包装和其他应用中的塑料替代品的潜力很大.
研究的目的:
- 为了调查Ulva fenestrata作为Ulvan的来源.
- 为优化的酶辅助和超声辅助提取方法.
- 评估提取时间和方法对乌尔旺产量和特性的影响.
主要方法:
- 使用细胞酶 (Viscozyme L,Cellulysin) 和蛋白酶 (Neutrase 0.8L,Flavourzyme) 的酶辅助提取.
- 不同的提取时间 (3,6,17,20小时).
- 结合酶和超声波辅助提取.
主要成果:
- 提取时间的延长增加了乌尔瓦的产量,在20小时后 Cellulysin 达到 14.1% dw.
- 结合酶和超声波提取的结果是最高的ulvan,为17.9% dw.
- 乌尔瓦芬斯特拉塔生物质含有高蛋白质含量 (19.8% dw),适用于食品和料.
结论:
- 乌尔瓦芬斯特拉塔 (Ulva fenestrata) 是一种可行的乌尔瓦提取源.
- 优化的酶和超声波辅助方法显著提高了乌尔万的产量.
- 剩余的生物质为蛋白质应用提供了有价值的副产品.
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