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海贝酸水解剂通过免疫调节和肠道微生物群恢复减轻小鼠DSS诱导的大肠炎
Hidayat Ullah1, Ting Deng1, Muhsin Ali1
1Department of Biotechnology, College of Basic Medical Science, Dalian Medical University, Dalian 116044, China.
Molecules (Basel, Switzerland)
|October 14, 2023
概括
海貝化水解劑 (CPH) 有效地治療乳硫酸在小鼠中的導致大腸炎. CPH缓解炎症,恢复肠道屏障功能,并重新平衡肠道微生物群,显示作为功能性食物和治疗剂的潜力.
科学领域:
- 胃肠病学 胃肠病学
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
背景情况:
- 炎症性肠病 (IBD) 涉及慢性消化系统炎症.
- 德克斯硫酸 (DSS) 诱导实验性结肠炎,导致上皮损伤和肠道失生症.
- 目前的IBD治疗包括益生菌,多糖和多.
研究的目的:
- 在小鼠模型中研究海贝化 (CPH) 对DSS诱导的大肠炎的治疗作用.
- 评估CPH对肠道炎症,上皮质完整性和肠道微生物群组成的影响.
主要方法:
- 在BALB/c小鼠中,使用2.5%的DSS在饮用水中诱导大肠炎,持续7天.
- 在DSS诱导后的14天内,小鼠接受了不同剂量的CPH (100,200,400毫克/公斤).
- 进行了组织学分析,细胞因子表达评估和16srRNA测序.
主要成果:
- CPH治疗显著降低了结肠炎的严重程度和症状.
- 皮质完整性得到改善,组织学损伤和炎症细胞透减少.
- CPH降低了促炎性细胞因子,通过上调紧接蛋白来恢复肠道屏障功能,并使肠道微生物群正常化,增加了Firmicutes和Lactobacillus.
结论:
- 海貝化水解劑 (CPH) 顯示了對DSS導致的大腸炎的重大治療潛力.
- CPH缓解肠道炎症,恢复肠道屏障的完整性和微生物群的平衡.
- CPH是功能性食品和用于胃肠道疾病的药物的有希望的候选者.
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