超临界二氧化碳用于提取Kleeb Bua Daeng公式的方法
Nittaya Ngamkhae1, Orawan Monthakantirat1, Yaowared Chulikhit1
1Division of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
Molecules (Basel, Switzerland)
|October 14, 2023
概括
超临界流体提取 (SFE) 有效地从Kleeb Bua Daeng (KBD) 草药配方中恢复植物化学物质. 优化的SFE条件产生了大量的,黄和胡卜素,证明了其绿色技术的潜力.
科学领域:
- 植物化学 植物化学
- 绿色化学 绿色化学
- 草药提取 草药提取 草药提取
背景情况:
- 超临界流体提取 (SFE) 是一种先进的,环保的技术,用于从植物材料中分离生物活性化合物.
- 泰国草药配方Kleeb Bua Daeng (KBD) 含有各种植物化学物质,具有潜在的健康益处.
- 优化SFE参数对于最大限度地回收所需化合物至关重要.
研究的目的:
- 评估SFE在从KBD配方中提取植物化学物质的有效性.
- 为了优化SFE条件 (时间,温度,压力) 用于KBD提取.
- 量化提取产量和关键植物化学物质的含量 (,黄,胡卜素,氨酸).
主要方法:
- 采用了一个盒子-Behnken设计 (BBD) 与响应表面方法 (RSM) 相结合.
- 研究的提取参数包括时间 (30-90分钟),温度 (30-60°C) 和压力 (200-300bar).
- 测量的响应变量是提取产量,总含量 (TPC),总黄含量 (TFC),总胡卜素含量 (TCC) 和总素含量 (TAC).
主要成果:
- 在60°C,300 bar和60分钟时获得了最高的提取产量 (3.81%).
- 为了最大限度地提高TPC,TFC和TCC的最佳条件是60°C,250 bar和90分钟.
- 没有检测到安托西亚宁,这表明SFE适用于低至中等极性化合物.
结论:
- SFE-CO2是一种高效和绿色的方法,可以从KBD草药配方中提取有价值的植物化学物质.
- 优化的SFE参数显著提高了,黄和胡卜素的恢复.
- 这项研究验证了SFE作为从KBD中提取植物化学物质的可持续方法,不包括安东.
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