同时确定随机选择的植物二次代谢物和植物提取物的抗氧化和亲氧化活性
Tibor Maliar1, Mária Maliarová1, Marcela Blažková2,3
1Department of Chemistry and Environmnetal Sciences, Faculty of Natural Sciences, University of Ss. Cyril and Methodius in Trnava, Nám. J. Herdu 2, 917 01 Trnava, Slovakia.
Molecules (Basel, Switzerland)
|October 14, 2023
概括
这项研究引入了一种新的方法,可以同时测量抗氧化和亲氧化活性. 初步发现显示,某些化合物,如黄类,表现出亲氧化作用,而其他化合物,如儿茶素,表现出抗氧化作用.
科学领域:
- 生物化学 生物化学
- 分析化学 分析化学
背景情况:
- 氧化应激是由抗氧化剂和亲氧化剂之间的不平衡造成的.
- 虽然抗氧化作用得到了充分的研究,但亲氧化作用需要进一步研究.
- 现有的亲氧化剂评估方法是多种多样的.
研究的目的:
- 开发一种新的,同时在现场的方法来评估抗氧化和亲氧化活性.
- 使用FRAP测试用于双重活性测量.
- 为了促进具有主导抗氧化和亲氧化性质的化合物之间的区别.
主要方法:
- 开发了一种单个微板测定方法,用于同时在现场确定抗氧化剂和亲氧化剂活性.
- 该方法利用FRAP试验中的Fenton反应来产生亲氧化Fe2+离子.
- 这种快速选方法可以有效评估各种化合物和提取物.
主要成果:
- 初步结果表明,含有众多氧异原子的化合物,特别是像黄类的sp2杂交化合物,表现出主要的亲氧化作用.
- 相反,儿茶素,胡卜素和树和栗子叶的提取物表现出主要的抗氧化活性.
- 开发的方法成功地区分了具有普遍抗氧化或亲氧化特征的化合物.
结论:
- 这种基于FRAP的新方法可以同时评估抗氧化和亲氧化活性.
- 特定的化学结构,如黄类,与主导的亲氧化作用有关.
- 需要进一步的系统研究来探索使用这种技术的更广泛的化合物和植物提取物.
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