对饮用水和自然水中的Sb3+离子进行敏感银增强微板apta酶检测
Nadezhda S Komova1, Kseniya V Serebrennikova1, Anna N Berlina1
1A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, Russia.
Molecules (Basel, Switzerland)
|October 14, 2023
概括
一种新的apt-enzyme测定检测到水中的有毒反 (Sb3+). 银离子增强显著提高了灵敏度,使得可以准确监测饮用水中的反水平.
科学领域:
- 分析化学 分析化学
- 环境科学 环境科学
- 生物技术是生物技术.
背景情况:
- 反 (Sb3+) 毒性需要可靠的水源监测方法.
- 现有的反检测分析技术可能很复杂或缺乏灵敏度.
- 简单,敏感的测试对于广泛的水质评估至关重要.
研究的目的:
- 开发一种敏感的微板apt-enzyme测定,用于Sb3+检测.
- 用银离子 (Ag+) 来提高试验的灵敏度和检测极限.
- 为了验证增强测试对现实世界水样的实际适用性.
主要方法:
- 开发一种微板apt-enzyme测定方法,使用生物化aptamer (A10) 和补充性寡核酸 (T10).
- 在微板井上的阿普坦 - 寡核酸复合物的固定,并通过胡卜过氧化酶 (HRP) 酶活性检测.
- 加入银离子 (Ag+) 来促进亚胺脱杂并增强Sb3+的结合.
主要成果:
- 最初的apt-enzyme测定检测到Sb3+,工作范围为0.092.3 μg/mL,检测极限为42 ng/mL.
- 用Ag+增强的试验在8135 ng/mL的工作范围内实现了显著较低的检测极限1.9 ng/mL.
- 增强分析显示了高选择性和成功应用在分析饮用水和泉水样本的高回收率.
结论:
- 开发的Ag+增强微板apt-enzyme试验为水中Sb3+检测提供了高度敏感和选择性的方法.
- 这种测试为水生环境中反污染的常规监测提供了有价值的工具.
- 增强的灵敏度和实际应用性使其适用于公共卫生和环境安全评估.
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